PMID- 9596484
OWN - NLM
STAT- MEDLINE
DCOM- 19980806
LR  - 20190905
IS  - 0167-6806 (Print)
IS  - 0167-6806 (Linking)
VI  - 48
IP  - 2
DP  - 1998 Mar
TI  - Aneuploidy of chromosome 8 as detected by interphase fluorescence in situ 
      hybridization is a recurrent finding in primary and metastatic breast cancer.
PG  - 125-33
AB  - Previous work from our laboratory demonstrated aneuploidy for several chromosomes 
      by interphase fluorescence in situ hybridization (FISH) in a high proportion of 
      breast cancer specimens. In the literature, only limited data are available 
      concerning chromosome 8 anomalies in breast cancer. To determine chromosome 8 
      ploidy status in primary and metastatic specimens from 81 breast cancer patients, 
      FISH analysis with a DNA probe recognizing chromosome 8 centromeres was 
      performed. In all primary tumor specimens (n = 30), significant proportions of 
      cells were aneuploid exhibiting gain of chromosome 8 copy numbers; in 75% of 
      effusion specimens previously classified as malignant by cytology and/or FISH for 
      various chromosomes (n = 40), cell populations aneuploid for chromosome 8 were 
      detected; effusions previously classified non-malignant (n = 11) were diploid in 
      10 cases, whereas one specimen contained rare hyperdiploid cells. Among these 
      cells complex chromosomal aneuploidy could be demonstrated by two-color FISH, 
      suggesting malignancy. Trisomic and tetrasomic clones were predominant in the 
      majority of samples, but a marked intratumor cytogenetic heterogeneity was 
      observed in most cases. Primary tumors and corresponding positive axillary lymph 
      nodes revealed similar distributions of chromosome 8 copy numbers, analogous to 
      previous findings with other chromosomes. This implies that, by using suitable 
      FISH probes after examination of the respective primary tumor, an efficient 
      search for (micro)metastasis might be feasible.
FAU - Roka, S
AU  - Roka S
AD  - First Department of Internal Medicine, University of Vienna, Austria.
FAU - Fiegl, M
AU  - Fiegl M
FAU - Zojer, N
AU  - Zojer N
FAU - Filipits, M
AU  - Filipits M
FAU - Schuster, R
AU  - Schuster R
FAU - Steiner, B
AU  - Steiner B
FAU - Jakesz, R
AU  - Jakesz R
FAU - Huber, H
AU  - Huber H
FAU - Drach, J
AU  - Drach J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Breast Cancer Res Treat
JT  - Breast cancer research and treatment
JID - 8111104
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Aneuploidy
MH  - Breast Neoplasms/*pathology/ultrastructure
MH  - Carcinoma, Ductal, Breast/*pathology/ultrastructure
MH  - *Chromosomes, Human, Pair 8
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Middle Aged
MH  - Neoplasm Metastasis
EDAT- 1998/05/22 00:00
MHDA- 1998/05/22 00:01
CRDT- 1998/05/22 00:00
PHST- 1998/05/22 00:00 [pubmed]
PHST- 1998/05/22 00:01 [medline]
PHST- 1998/05/22 00:00 [entrez]
AID - 10.1023/a:1005937305102 [doi]
PST - ppublish
SO  - Breast Cancer Res Treat. 1998 Mar;48(2):125-33. doi: 10.1023/a:1005937305102.