PMID- 9596929
OWN - NLM
STAT- MEDLINE
DCOM- 19980701
LR  - 20061115
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 77
IP  - 4
DP  - 1997 Apr
TI  - [The role of NADPH in the development of neonatal jaundice with G6PD deficiency].
PG  - 278-81
AB  - OBJECTIVE: To investigate the role of NADPH in the development of neonatal 
      jaundice with G6PD deficiency. METHODS: The enzyme activities of G6PD, catalse 
      (Cat) and glutathione peroxidase (GSH-px) were measured by quantitative 
      determination of enzyme activity. The level of MDA was analyzed with 
      alpha-thiobarbituric acid and the level of NADPH was determined with modified 
      Nisselbaum JS's. Comparisons of these markers between G6PD normal and deficient 
      erythrocytes were made before and during the incubation of the erythrocytes with 
      H2O2. RESULTS: The level of MDA, which was 36 +/- 8n-mol.L-1.gHb-1, was increased 
      and that of NADPH, which was 1792 +/- 106mumol.L-1.gHb-1, was decreased in 
      jaundiced neonates with G6PD deficiency compared with those with normal G6PD 
      activity. When the cells were incubated with H2O2, the level of NADPH and the 
      activities of Cat and GSHpx in erythrocytes with normal G6PD activity increased 
      at first, and then turned to decrease as the incubation lasted longer than 30 
      minutes. But in G6PD-deficient erythrocytes all these markers decreased 
      continuously as the cells were incubated with H2O2. CONCLUSIONS: The diminished 
      capability of generation of NADPH in G6PD-deficient erythrocytes may contribute 
      directly to the more extensive peroxidation of the cells. The defect capacity of 
      generation of NADPH, which resulted in the weakened capability of 
      antiperoxidation and finally the lysis of erythrocytes, was one of the important 
      mechanisms in the development of jaundice in G6PD-deficient neonates.
FAU - Chen, F
AU  - Chen F
AD  - Pediatric Department, First Teaching Hospital of Guangzhou Medical College.
FAU - Zhang, Y
AU  - Zhang Y
FAU - Wu, Z
AU  - Wu Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 53-59-8 (NADP)
SB  - IM
MH  - Glucosephosphate Dehydrogenase Deficiency/complications/*enzymology
MH  - Humans
MH  - Infant, Newborn
MH  - Jaundice, Neonatal/*enzymology/etiology
MH  - NADP/*blood
EDAT- 1997/04/01 00:00
MHDA- 1998/05/23 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1998/05/23 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 1997 Apr;77(4):278-81.