PMID- 9598300
OWN - NLM
STAT- MEDLINE
DCOM- 19980805
LR  - 20190116
IS  - 0148-0545 (Print)
IS  - 0148-0545 (Linking)
VI  - 21
IP  - 2
DP  - 1998 May
TI  - Contact hypersensitivity to dicyclohexylcarbodiimide and diisopropylcarbodiimide 
      in female B6C3F1 mice.
PG  - 195-206
AB  - Dicyclohexylcarbodiimide (DCC) and diisopropylcarbodiimide (DIC) are two commonly 
      used coupling reagents in protein synthesis resulting in exposure of individuals 
      in chemical and pharmaceutical industries as well as research laboratories 
      involved in protein synthesis and recombinant DNA techniques. The objectives of 
      these studies were to determine the irritation and sensitizing potential of these 
      two compounds when applied topically to B6C3F1 mice. Sensitization potential was 
      assessed by the Mouse Ear Swelling Test (MEST) and the murine Local Lymph Node 
      Assay (LLNA). Concentrations used in the contact hypersensitivity assays were 
      determined by primary irritancy studies. DCC and DIC were identified as both 
      irritants and contact sensitizers with the MEST being a more sensitive indicator 
      of sensitization potential. The MEST identified DCC as a sensitizer at 
      concentrations as low as 0.006% (w/v) 24 hr and 48 hr post challenge and DIC at 
      0.3% (w/v) and 1.5% (w/v) 24 and 48 hr post challenge, respectively. In the LLNA, 
      the lowest concentrations yielding a significant response were 0.06% (w/v) for 
      DCC and 10% (w/v) for DIC.
FAU - Hayes, B B
AU  - Hayes BB
AD  - Department of Pharmacology and Toxicology, Medical College of Virginia, 
      Commonwealth University, Richmond 23298, USA.
FAU - Gerber, P C
AU  - Gerber PC
FAU - Griffey, S S
AU  - Griffey SS
FAU - Meade, B J
AU  - Meade BJ
LA  - eng
GR  - ES05288/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Drug Chem Toxicol
JT  - Drug and chemical toxicology
JID - 7801723
RN  - 0 (Carbodiimides)
RN  - 0 (Irritants)
RN  - 538-75-0 (Dicyclohexylcarbodiimide)
RN  - 693-13-0 (1,3-diisopropylcarbodiimide)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Carbodiimides/administration & dosage/*toxicity
MH  - Dermatitis, Contact/*etiology
MH  - Dicyclohexylcarbodiimide/administration & dosage/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Ear
MH  - Female
MH  - Irritants/administration & dosage/*toxicity
MH  - Lymph Nodes/drug effects/pathology
MH  - Mice
EDAT- 1998/05/23 00:00
MHDA- 1998/05/23 00:01
CRDT- 1998/05/23 00:00
PHST- 1998/05/23 00:00 [pubmed]
PHST- 1998/05/23 00:01 [medline]
PHST- 1998/05/23 00:00 [entrez]
AID - 10.3109/01480549809011647 [doi]
PST - ppublish
SO  - Drug Chem Toxicol. 1998 May;21(2):195-206. doi: 10.3109/01480549809011647.