PMID- 9598797
OWN - NLM
STAT- MEDLINE
DCOM- 19980721
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 22
IP  - 2
DP  - 1998 Jun
TI  - Molecular cytogenetic analysis of consistent abnormalities at 8q12-q22 in breast 
      cancer.
PG  - 105-13
AB  - Studies using comparative genomic hybridization (CGH) indicate that portions of 
      chromosome arm 8q from 8q12 to 8qter are present at an increased relative copy 
      number in a broad range of solid tumors. In this study we define an approximately 
      1 Mb wide region that appears to be frequently abnormal in copy number or 
      structure in breast cancer cell lines and primary tumors. This was accomplished 
      by fluorescence in situ hybridization (FISH) with yeast artificial chromosomes 
      (YACs) mapped to 8q2-q22. Eleven breast cancer cell lines and ten primary tumors 
      were analyzed. A minimal region of rearrangement was localized to the CEPH-YAC 
      928F9 in three breast cancer cell lines with unbalanced translocation breakpoints 
      mapping in this region. Unbalanced translocations also were detected in two 
      primary tumors mapping between CEPH-YAC clones 890C4 and 936B3, flanking 928F9. 
      An increased copy number in the minimal region was detected in nine cell lines 
      and in multiple primary tumors. This suggests the possibility that a single gene 
      mapping to 928F9 is involved in breast cancer development or progression and may 
      be deregulated by copy number increases in some tumors and by translocation in 
      others. Four expressed sequence tags were mapped to YAC 928F9 and analyzed for 
      rearrangements by Southern analysis and for abnormal expression by Northern 
      analysis.
FAU - Fejzo, M S
AU  - Fejzo MS
AD  - UCSF Cancer Center, University of California, San Francisco 94115, USA.
FAU - Godfrey, T
AU  - Godfrey T
FAU - Chen, C
AU  - Chen C
FAU - Waldman, F
AU  - Waldman F
FAU - Gray, J W
AU  - Gray JW
LA  - eng
GR  - CA09215/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Chromosome Aberrations/diagnosis/*genetics
MH  - Chromosome Disorders
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - DNA Probes
MH  - Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Polymerase Chain Reaction
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1998/05/23 02:18
MHDA- 2000/06/20 09:00
CRDT- 1998/05/23 02:18
PHST- 1998/05/23 02:18 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/05/23 02:18 [entrez]
AID - 10.1002/(SICI)1098-2264(199806)22:2<105::AID-GCC4>3.0.CO;2-0 [pii]
AID - 10.1002/(sici)1098-2264(199806)22:2<105::aid-gcc4>3.0.co;2-0 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1998 Jun;22(2):105-13. doi: 
      10.1002/(sici)1098-2264(199806)22:2<105::aid-gcc4>3.0.co;2-0.