PMID- 9600233
OWN - NLM
STAT- MEDLINE
DCOM- 19980610
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 102
IP  - 4
DP  - 1998 Apr
TI  - Analysis of HLA-DQ alpha sequences for prenatal diagnosis in single fetal cells 
      from maternal blood.
PG  - 393-6
AB  - We have extended a previously developed method that allows prenatal DNA diagnosis 
      of female fetuses through the isolation of single nucleated erythrocytes from 
      maternal blood by developing a method that can distinguish between maternal and 
      fetal nucleated erythrocytes. Nucleated erythrocytes were separated by a 
      density-gradient method and then collected by micromanipulation. Sex was 
      determined after primer extension preamplification (PEP) of the entire genome of 
      a single cell, and human leukocyte antigen (HLA)-DQ alpha type was determined 
      after further amplification of this gene. The HLA-DQ alpha genotype of fetal 
      erythrocytes in maternal blood samples and their corresponding paternal and 
      maternal lymphocytes were successfully determined in all cases. The accuracy of 
      the method was determined by using single nucleated erythrocytes from umbilical 
      cord blood from five normal deliveries. This is the first demonstration that the 
      fetal HLA-DQ alpha gene sequences can be identified in a small aliquot of a 
      single nucleated erythrocyte in maternal blood. We believe that this method 
      ushers in a new era in which the reliability and accuracy of noninvasive prenatal 
      DNA diagnosis from maternal blood is markedly improved.
FAU - Sekizawa, A
AU  - Sekizawa A
AD  - Department of Obstetrics and Gynecology, School of Medicine, Showa University, 
      Shinagawa, Tokyo, Japan.
FAU - Taguchi, A
AU  - Taguchi A
FAU - Watanabe, A
AU  - Watanabe A
FAU - Kimura, T
AU  - Kimura T
FAU - Saito, H
AU  - Saito H
FAU - Yanaihara, T
AU  - Yanaihara T
FAU - Sato, T
AU  - Sato T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ alpha-Chains)
RN  - 0 (HLA-DQA1 antigen)
SB  - IM
MH  - Alleles
MH  - Erythroblasts/*chemistry
MH  - Female
MH  - Fetus
MH  - Genotype
MH  - HLA-DQ Antigens/*blood/*genetics
MH  - HLA-DQ alpha-Chains
MH  - Histocompatibility Testing
MH  - Humans
MH  - Male
MH  - Maternal-Fetal Exchange/genetics
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
EDAT- 1998/05/26 00:00
MHDA- 1998/05/26 00:01
CRDT- 1998/05/26 00:00
PHST- 1998/05/26 00:00 [pubmed]
PHST- 1998/05/26 00:01 [medline]
PHST- 1998/05/26 00:00 [entrez]
AID - 10.1007/s004390050710 [doi]
PST - ppublish
SO  - Hum Genet. 1998 Apr;102(4):393-6. doi: 10.1007/s004390050710.