PMID- 9604763
OWN - NLM
STAT- MEDLINE
DCOM- 19980818
LR  - 20190515
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 15
IP  - 5
DP  - 1998 May
TI  - Incidence of chromosomal abnormalities from a morphologically normal cohort of 
      embryos in poor-prognosis patients.
PG  - 297-301
AB  - PURPOSE: Preimplantation genetic diagnosis of aneuploidy was performed on the 
      embryos yielded by 70 poor-prognosis patients, with the aim of transferring those 
      with a normal chromosomal complement, thus possibly increasing the chances of 
      pregnancy. METHODS: Multicolor fluorescence in situ hybridization (FISH) was 
      applied for the simultaneous detection of chromosomes X, Y, 13, 16, 18, and 21. 
      Inclusion criteria were (1) a maternal age of 36 years or older (n = 33), (2) 
      three or more previous in vitro fertilization cycles (n = 20), and (3) an altered 
      karyotype (n = 17). RESULTS: A total of 412 embryos underwent FISH, resulting in 
      234 (57%) that were chromosomally abnormal. Euploid embryos were available for 
      transfer in 59 patients, generating 19 pregnancies (32%), with an implantation 
      rate of 19.9%. CONCLUSIONS: High rates of chromosomally abnormal embryos in 
      poor-prognosis patients can determine repeated in vitro fertilization failures 
      when embryo selection is performed on the basis of morphological criteria alone. 
      Hence, the FISH analysis could represent the prevailing approach for the 
      identification of embryos possessing full potential for developing to term.
FAU - Magli, M C
AU  - Magli MC
AD  - S.I.S.ME.R., Reproductive Medicine Unit, Bologna, Italy.
FAU - Gianaroli, L
AU  - Gianaroli L
FAU - Munne, S
AU  - Munne S
FAU - Ferraretti, A P
AU  - Ferraretti AP
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - Chromosome Aberrations/*diagnosis/genetics/pathology
MH  - Chromosome Disorders
MH  - Embryo, Mammalian/chemistry/pathology/*physiology
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - Pregnancy, High-Risk/*genetics
MH  - *Preimplantation Diagnosis
MH  - Prognosis
PMC - PMC3454748
EDAT- 1998/05/30 00:00
MHDA- 1998/05/30 00:01
PMCR- 1999/05/01
CRDT- 1998/05/30 00:00
PHST- 1998/05/30 00:00 [pubmed]
PHST- 1998/05/30 00:01 [medline]
PHST- 1998/05/30 00:00 [entrez]
PHST- 1999/05/01 00:00 [pmc-release]
AID - 421539 [pii]
AID - 10.1023/a:1022596528036 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 1998 May;15(5):297-301. doi: 10.1023/a:1022596528036.