PMID- 9604767
OWN - NLM
STAT- MEDLINE
DCOM- 19980818
LR  - 20190515
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 15
IP  - 5
DP  - 1998 May
TI  - Simultaneous detection of chromosomes X, Y, 13, 18, and 21 by fluorescence in 
      situ hybridization in blastomeres obtained from preimplantation embryos.
PG  - 314-9
AB  - PURPOSE: Simultaneous fluorescence in situ hybridization (FISH) was used in a 
      preimplantation genetic diagnosis program to determine which embryos were normal 
      for chromosomes X, Y, 13, 18, and 21. METHODS: Single blastomeres were disrupted 
      and attached to glass slides using acetic acid and ethanol. Using a ratio mixture 
      of chromosome enumeration DNA probes in combination with locus-specific 
      identifier DNA probes, FISH was performed for the identification of chromosomes 
      X, Y, 13, 18, and 21. RESULTS: Fourteen couples enrolled in IVF produced 134 
      embryos for biopsy. Blastomeres subjected to five-color FISH revealed that 22% of 
      embryos were normal for chromosomes X, Y, 13, 18, and 21. In addition, 52% were 
      abnormal and no results could be detected for 25%. Twelve couples underwent 
      embryo transfer, two couples did not receive embryos due to lack of any normal 
      embryos, and three couples became pregnant. CONCLUSIONS: The simultaneous 
      detection of five-color FISH is a feasible method to detect aneuploidy in 
      preimplantation embryos from women of advanced maternal age.
FAU - Smith, S E
AU  - Smith SE
AD  - Reproductive Biology Associates, Atlanta, Georgia 30342, USA.
FAU - Toledo, A A
AU  - Toledo AA
FAU - Massey, J B
AU  - Massey JB
FAU - Kort, H I
AU  - Kort HI
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Adult
MH  - Blastomeres/*pathology
MH  - Chromosome Aberrations/diagnosis/genetics
MH  - Chromosome Disorders
MH  - Chromosomes, Human/*genetics
MH  - Chromosomes, Human, Pair 13/genetics
MH  - Chromosomes, Human, Pair 18/genetics
MH  - Chromosomes, Human, Pair 21/genetics
MH  - DNA Probes
MH  - Female
MH  - Fertilization in Vitro
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Pregnancy
MH  - X Chromosome/genetics
MH  - Y Chromosome/genetics
PMC - PMC3454756
EDAT- 1998/05/30 00:00
MHDA- 1998/05/30 00:01
PMCR- 1999/05/01
CRDT- 1998/05/30 00:00
PHST- 1998/05/30 00:00 [pubmed]
PHST- 1998/05/30 00:01 [medline]
PHST- 1998/05/30 00:00 [entrez]
PHST- 1999/05/01 00:00 [pmc-release]
AID - 421543 [pii]
AID - 10.1023/a:1022504829854 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 1998 May;15(5):314-9. doi: 10.1023/a:1022504829854.