PMID- 9605187
OWN - NLM
STAT- MEDLINE
DCOM- 19980619
LR  - 20061115
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 78
IP  - 5
DP  - 1998 May
TI  - Different susceptibility of mice to immune-mediated cholangitis induced by 
      immunization with carbonic anhydrase II.
PG  - 629-37
AB  - Carbonic anhydrase II (CA-II), an enzyme that catalyzes hydration of carbon 
      dioxide to bicarbonate and hydrogen ions, is located exclusively in 
      cholangiocytes in the liver. Recently, patients with autoimmune cholangitis have 
      been reported to have serum antibodies to CA-II. Moreover, active immunization of 
      susceptible mice with CA-II results in inflammation of submandibular glands, 
      where CA-II is also expressed. In the present study, we attempted to produce 
      cholangitis by immunization with CA-II using two strains of mice with different 
      potential susceptibilities. Balb/c and DBA/1J mice were immunized with a dose of 
      human CA-II (100 microg) intraperitoneally every other week on three occasions. 
      One week after the final immunization, mice were killed and blood and tissue 
      samples harvested. Light and electron microscopic evaluation for inflammation was 
      performed under coded identification. After immunization of Balb/c mice, numerous 
      mononuclear cells, mostly CD4-positive T cells, appeared around bile ducts; 
      lymphocyte invasion between cholangiocytes was also seen. Inflammation was not 
      observed outside the liver. Morphologic evidence of cholangitis was observed in 8 
      (53.3%) of 15 Balb/c mice and in 3 (20%) of 15 DBA/1 J mice. In the control mice 
      immunized with bovine serum albumin (BSA), cholangitis was observed in only 1 
      (6.7%) of 15 Balb/c mice and none of 15 DBA/1J mice. Balb/c mice immunized with 
      CA-II had statistically significant cholangitis compared with those immunized 
      with BSA (p < 0.01), whereas DBA/1J did not show a significant difference from 
      controls. Balb/c mice immunized with CA-II showed specific antibody production 
      after immunization, whereas DBA/1J mice immunized with CA-II had anti-CA-II 
      antibody even in preimmune sera. Adoptive transfer of splenocytes from 
      CA-II-immunized Balb/c mice resulted in cholangitis in two (66.7%) of three 
      Balb/c recipients. These data strongly suggest that the cholangitis can be 
      induced by CA-II immunization in susceptible strains of mice.
FAU - Ueno, Y
AU  - Ueno Y
AD  - Third Department of Internal Medicine, Tohoku University School of Medicine, 
      Sendai, Japan.
FAU - Ishii, M
AU  - Ishii M
FAU - Takahashi, S
AU  - Takahashi S
FAU - Igarashi, T
AU  - Igarashi T
FAU - Toyota, T
AU  - Toyota T
FAU - LaRusso, N F
AU  - LaRusso NF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Enzymes)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Carbonic Anhydrases/*immunology
MH  - Cholangitis/*immunology/metabolism/pathology
MH  - Disease Susceptibility
MH  - Enzymes/blood
MH  - *Immunization
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Liver/enzymology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred DBA
MH  - Microscopy, Electron
EDAT- 1998/05/30 00:00
MHDA- 1998/05/30 00:01
CRDT- 1998/05/30 00:00
PHST- 1998/05/30 00:00 [pubmed]
PHST- 1998/05/30 00:01 [medline]
PHST- 1998/05/30 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1998 May;78(5):629-37.