PMID- 9605521
OWN - NLM
STAT- MEDLINE
DCOM- 19980727
LR  - 20201209
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 137
IP  - 2
DP  - 1998 Feb
TI  - Prolactin induces expression of FGF-2 and a novel FGF-responsive 
      NonO/p54nrb-related mRNA in rat lymphoma cells.
PG  - 187-95
AB  - The rat Nb2-11C lymphoma cell line expresses high affinity prolactin (PRL) 
      receptors, and requires lactogenic hormones for survival and proliferation. We 
      have applied differential display to identify genes which are differentially 
      induced in Nb2-11C cells following PRL stimulation, or which are constitutively 
      expressed in the PRL-independent Nb2-Sp cells. In the present study we 
      characterized a clone (22c.2) which was expressed in Nb2-Sp cells, and in Nb2-11C 
      cells given PRL for 3 h but not in untreated cells. The 279 bp cDNA had 95% 
      homology with the 3' end of the murine 2.6 kb FGF-inducible gene 14 (FIN14). When 
      clone 22c.2 was used to screen a Nb2-Sp cDNA library to obtain a longer cDNA, a 
      unique 1039 bp clone PNR (Prolactin-responsive/ NonO-Related) was isolated, 
      subcloned and sequenced. The deduced amino acid sequence encoded by the PNR open 
      reading frame had significant homology with a family of RNA- and DNA-binding 
      proteins which include the human polypyrimidine tract-binding protein 
      (PTB)-associated splicing factor (PSF), the murine non-POU-domain-containing 
      octamer-binding protein (NonO) and the human NonO homologue p54nrb. Nb2-11C cells 
      expressed three PNR-related mRNA transcripts of 2.5, 3.0 and > 10 kb. Expression 
      of the 2.5 and 3.0 kb transcripts were increased at least 4-fold within 3 h of 
      PRL treatment. PNR expression was also significantly stimulated within 3 h by 
      addition of FGF-2 to either Nb2-11C or Nb2-Sp cells, although alone FGF-2 was not 
      mitogenic for either cell line. Reverse transcription-polymerase chain reaction 
      (RT-PCR) confirmed the expression of both FGF-2 and FGF receptor mRNA in Nb2 
      cells. raising the possibility of an autocrine or paracrine function for FGF-2 in 
      lymphoma cells. Furthermore, PRL rapidly stimulated the expression of FGF-2 mRNA 
      in a time- and dose-dependent manner in both Nb2-11C and Nb2-Sp cells. FGF-2 
      expression was increased within 1 h and was maintained at a high level for at 
      least 10 h following treatment with 2 ng/ml PRL. Western blotting with anti-FGF2 
      antisera demonstrated PRL stimulation of intracellular accumulation, but not 
      secretion of immunoreactive FGF-2. The observation of PRL-responsive expression 
      of FGF-2 in Nb2 cells suggests a previously unrecognized pathway for PRL action 
      in lymphoid cells.
FAU - Too, C K
AU  - Too CK
AD  - Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada. 
      CTOO@IS.DAL.CA
FAU - Knee, R
AU  - Knee R
FAU - Pinette, A L
AU  - Pinette AL
FAU - Li, A W
AU  - Li AW
FAU - Murphy, P R
AU  - Murphy PR
LA  - eng
SI  - GENBANK/AF036335
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NONO protein, human)
RN  - 0 (Nuclear Matrix-Associated Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Octamer Transcription Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (RNA-Binding Proteins)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 9002-62-4 (Prolactin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics/isolation & purification
MH  - DNA-Binding Proteins
MH  - Fibroblast Growth Factor 2/biosynthesis/*genetics/pharmacology
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Lymphocytes/drug effects/metabolism
MH  - Lymphoma/genetics/metabolism
MH  - Molecular Sequence Data
MH  - *Nuclear Matrix-Associated Proteins
MH  - Nuclear Proteins/*genetics
MH  - Octamer Transcription Factors
MH  - Polymerase Chain Reaction
MH  - Prolactin/*pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Neoplasm/genetics/metabolism
MH  - RNA-Binding Proteins/*genetics
MH  - Rats
MH  - Tumor Cells, Cultured
EDAT- 1998/05/30 00:00
MHDA- 1998/05/30 00:01
CRDT- 1998/05/30 00:00
PHST- 1998/05/30 00:00 [pubmed]
PHST- 1998/05/30 00:01 [medline]
PHST- 1998/05/30 00:00 [entrez]
AID - S0303-7207(97)00240-2 [pii]
AID - 10.1016/s0303-7207(97)00240-2 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1998 Feb;137(2):187-95. doi: 10.1016/s0303-7207(97)00240-2.