PMID- 9606906
OWN - NLM
STAT- MEDLINE
DCOM- 19980623
LR  - 20061115
IS  - 0008-4166 (Print)
IS  - 0008-4166 (Linking)
VI  - 44
IP  - 3
DP  - 1998 Mar
TI  - Functional and structural successions in arbitrary samples of heterotrophic 
      bacteria during aerobic treatments of lignite-carbonization wastewater in in situ 
      enclosures.
PG  - 211-20
AB  - In situ mesocosm experiments were performed in Lake Schwelvollert (located in the 
      district of Weissenfels, Saxony-Anhalt, Germany), an anaerobic 
      lignite-carbonization effluent lake containing phenolic compounds and their 
      autoxidation products (anthropogenic humic matter). In the aeration enclosure, 
      the anaerobic Schwelvollert wastewater was aerated and in the flocculation 
      enclosure, it was flocculated to precipitate the oxygen-trapping anthropogenic 
      humic matter to enhance the input of oxygen by diffusion. To gain an insight into 
      the metabolic state of the aerobic heterotrophic microbiota during the 
      treatments, arbitrary samples of bacterial isolates were taken from a general 
      agar medium and tested for their abilities to cleave predominant phenolic 
      contaminants by a procedure called the isolate sample assay. In this way, 
      successions of degradation potentials were observed in both mesocosms, with 
      degradation abilities for meta- and para-alkylated phenols appearing before 
      degradation abilities for ortho-substituted phenols as a common phenomenon. To 
      examine the structure of samples, the respective isolates were characterized 
      using the Biolog GN MicroPlate system, the random amplified polymorphic DNA 
      nucleic acid (RAPD) fingerprinting technique, and amplified ribosomal DNA 
      restriction analysis (ARDRA). Although similar functional patterns occurred in 
      both mesocosms, the compositions and diversities of the respective bacterial 
      communities varied significantly, even at different depths from the same 
      enclosure, with members of the Pseudomonas RNA group I being predominant.
FAU - Becker, P M
AU  - Becker PM
AD  - Department of Remediation Research, UFZ Centre for Environmental Research 
      Leipzig-Halle, Germany. becker@san.ufz.de
FAU - Wand, H
AU  - Wand H
FAU - Martius, G G
AU  - Martius GG
FAU - Weissbrodt, E
AU  - Weissbrodt E
FAU - Stottmeister, U
AU  - Stottmeister U
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Can J Microbiol
JT  - Canadian journal of microbiology
JID - 0372707
RN  - 0 (Industrial Waste)
RN  - 0 (Phenols)
SB  - IM
MH  - Aerobiosis
MH  - Bacteria, Aerobic/classification/genetics/isolation & purification/*physiology
MH  - Biodegradation, Environmental
MH  - Cluster Analysis
MH  - Colony Count, Microbial
MH  - DNA Fingerprinting
MH  - Ecosystem
MH  - *Industrial Waste
MH  - Phenols/*metabolism
MH  - Pseudomonas/genetics/isolation & purification/physiology
MH  - Random Amplified Polymorphic DNA Technique
MH  - *Water Microbiology
MH  - *Water Pollution, Chemical
EDAT- 1998/06/02 00:00
MHDA- 1998/06/02 00:01
CRDT- 1998/06/02 00:00
PHST- 1998/06/02 00:00 [pubmed]
PHST- 1998/06/02 00:01 [medline]
PHST- 1998/06/02 00:00 [entrez]
PST - ppublish
SO  - Can J Microbiol. 1998 Mar;44(3):211-20.