PMID- 9606920
OWN - NLM
STAT- MEDLINE
DCOM- 19980612
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 81
IP  - 3
DP  - 1998 May-Jun
TI  - Determination of ivermectin in salmon muscle tissue by liquid chromatography with 
      fluorescence detection.
PG  - 549-53
AB  - A liquid chromatographic method was developed for determination of ivermectin B1a 
      (IVR) extracted from raw fortified and incurred Atlantic salmon muscle tissues. 
      The method was also used to determine fortified doramectin (DOR) in Atlantic 
      salmon. Tissue extract was applied to C8 solid-phase extraction (SPE) column, 
      followed by a silica SPE column. Residues in the eluate were treated with 
      trifluoroacetic anhydride and methylimidazole to dehydrate the IVR molecule and 
      form an aromatic fluorescent moiety with a trifluoroacetic ester. This product 
      was subsequently treated with ammonium acetate in methanol to cleave the ester 
      and convert the functional group back to a stable alcohol form. The analytes were 
      determined by fluorescence with excitation at 272 nm and emission at 465 nm. A 
      C18 Hypersil column was used for analysis with a mobile phase of 
      acetonitrile-water (90 + 10, v/v) and an oven temperature of 65 degrees C. IVR 
      and DOR were determined at 5 fortification levels (1, 5, 10, 20, and 40 ppb). 
      Intra-assay absolute recoveries ranged from 75 to 89% for IVR and from 73 to 85% 
      for DOR. Relative standard deviations (RSDs) were < 7% in all cases. The limit of 
      detection (3 x baseline noise) was 0.25 ppb extracted from tissue. Incurred 
      tissues had an average concentration of 32 ppb, with an RSD of 3%.
FAU - Rupp, H S
AU  - Rupp HS
AD  - U.S. Food and Drug Administration, Seattle District Office, Bothell, WA 
      98021-4421, USA.
FAU - Turnipseed, S B
AU  - Turnipseed SB
FAU - Walker, C C
AU  - Walker CC
FAU - Roybal, J E
AU  - Roybal JE
FAU - Long, A R
AU  - Long AR
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Insecticides)
RN  - 0 (Macrolides)
RN  - 0 (Pesticide Residues)
RN  - 70288-86-7 (Ivermectin)
RN  - KGD7A54H5P (doramectin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, Liquid/methods
MH  - Drug Stability
MH  - Insecticides/*analysis
MH  - Ivermectin/analogs & derivatives/*analysis
MH  - Macrolides
MH  - Muscles/*chemistry
MH  - Pesticide Residues/*analysis
MH  - Reproducibility of Results
MH  - Salmon/*metabolism
MH  - Sensitivity and Specificity
MH  - Spectrometry, Fluorescence
EDAT- 1998/06/02 00:00
MHDA- 1998/06/02 00:01
CRDT- 1998/06/02 00:00
PHST- 1998/06/02 00:00 [pubmed]
PHST- 1998/06/02 00:01 [medline]
PHST- 1998/06/02 00:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 1998 May-Jun;81(3):549-53.