PMID- 9609457
OWN - NLM
STAT- MEDLINE
DCOM- 19980720
LR  - 20180213
IS  - 1660-8151 (Print)
IS  - 1660-8151 (Linking)
VI  - 79
IP  - 1
DP  - 1998
TI  - Detection of apoptotic cells in glomeruli of patients with IgA nephropathy.
PG  - 21-7
AB  - The purpose of the present study was to determine the relationship between the 
      existence of apoptotic cells in glomeruli, clinical or histopathological findings 
      and response to treatment in patients with IgA nephropathy. Renal biopsy 
      specimens were obtained from 23 patients with IgA nephropathy. These patients 
      were divided into two groups: mild glomerular damage (12 patients), and severe 
      glomerular damage (11 patients). The nick end-labelling method (TUNEL) and 
      fluorescent staining (Hoechst 33258) were used for the detection of apoptotic 
      cells. Five of 6 patients with apoptotic cells in the glomeruli detected by TUNEL 
      were in the severe glomerular damage group, and only 1 patient was in the mild 
      glomerular damage group. Apoptotic cells in glomeruli were also detected by 
      fluorescent staining in 3 of 5 patients in the severe glomerular damage group who 
      showed apoptotic cells in TUNEL. However, no apoptotic cells were detected in 
      patients in the mild glomerular damage group in fluorescent staining. Mean levels 
      of urinary protein excretion at the time of renal biopsy in the patients with 
      apoptotic cells were significantly higher than those in patients without 
      apoptotic cells (p < 0.01). The mean levels of creatinine clearance (Ccr) in the 
      patients with apoptotic cells were slightly lower than those in patients without 
      such cells. There were no significant differences in the levels of serum 
      creatinine (s-Cr) and BUN in patients with or without apoptotic cells. In the 
      severe glomerular damage group, urinary protein excretion after treatment in the 
      patients with apoptotic cells was significantly improved compared with that in 
      the patients without such cells (p < 0.01). It appears that the levels of 
      proteinuria and renal function tests might be influenced by apoptosis in patients 
      with IgA nephropathy. It is postulated that apoptosis may induce reduction of 
      excess proliferative mesangial cells and/or infiltrated cells, and tissue repair. 
      Thereafter, these histological alterations may improve proteinuria and renal 
      function.
FAU - Tashiro, K
AU  - Tashiro K
AD  - Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
FAU - Kodera, S
AU  - Kodera S
FAU - Takahashi, Y
AU  - Takahashi Y
FAU - Horikoshi, S
AU  - Horikoshi S
FAU - Shirato, I
AU  - Shirato I
FAU - Tomino, Y
AU  - Tomino Y
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Nephron
JT  - Nephron
JID - 0331777
RN  - AYI8EX34EU (Creatinine)
RN  - EC 2.7.7.31 (DNA Nucleotidylexotransferase)
SB  - IM
MH  - Adult
MH  - Apoptosis/*physiology
MH  - Biopsy
MH  - Creatinine/blood
MH  - DNA Nucleotidylexotransferase/metabolism
MH  - Female
MH  - Glomerulonephritis, IGA/*physiopathology
MH  - Histocytochemistry
MH  - Humans
MH  - Kidney Function Tests
MH  - Kidney Glomerulus/*pathology
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Proteinuria/pathology
EDAT- 1998/06/03 02:04
MHDA- 2000/10/06 11:01
CRDT- 1998/06/03 02:04
PHST- 1998/06/03 02:04 [pubmed]
PHST- 2000/10/06 11:01 [medline]
PHST- 1998/06/03 02:04 [entrez]
AID - nef79021 [pii]
AID - 10.1159/000044986 [doi]
PST - ppublish
SO  - Nephron. 1998;79(1):21-7. doi: 10.1159/000044986.