PMID- 9609459
OWN - NLM
STAT- MEDLINE
DCOM- 19980720
LR  - 20180213
IS  - 1660-8151 (Print)
IS  - 1660-8151 (Linking)
VI  - 79
IP  - 1
DP  - 1998
TI  - A high glucose concentration stimulates the expression of monocyte chemotactic 
      peptide 1 in human mesangial cells.
PG  - 33-7
AB  - The mechanism of glomerular infiltration of monocytes remains unknown in diabetic 
      nephropathy. We examined the effect of a high glucose concentration on monocyte 
      chemotactic peptide 1 (MCP-1) expression in human mesangial cells (MCs) by using 
      enzyme-linked immunosorbent assay and reverse transcription coupled with 
      polymerase chain reaction (PCR). More than a 50% increase in the MCP-1 protein 
      production was observed in MCs cultured in high-glucose medium (450 mg/dl) as 
      compared to normal glucose (100 mg/dl; 1,496 +/- 75 vs. 966 +/- 15 pg/ml after 24 
      h, 1,910 +/- 93 vs. 1,250 +/- 55 pg/ml after 48 h). Semiquantitative PCR showed 
      that phorbol myristate acetate (100 nM) increased the ratio of PCR products for 
      MCP-1 to housekeeping gene glyceraldehyde-3-phosphate dehydrogenase on 
      densitometric results at 24 h by 2.7-fold, which was prevented by calphostin C 
      (200 nM) pretreatment. High glucose increased the ratio by 3-fold as compared to 
      normal glucose at 24 h (0.72 +/- 0.11 vs. 0.24 +/- 0.01). This was also 
      suppressed by calphostin C pretreatment. These findings demonstrate that high 
      glucose can directly increase MCP-1 expression in MCs, which may contribute to 
      monocyte infiltration in diabetic nephropathy, and this is regulated by protein 
      kinase C.
FAU - Ihm, C G
AU  - Ihm CG
AD  - Department of Internal Medicine, College of Medicine, Kyung Hee University, 
      Seoul, Korea.
FAU - Park, J K
AU  - Park JK
FAU - Hong, S P
AU  - Hong SP
FAU - Lee, T W
AU  - Lee TW
FAU - Cho, B S
AU  - Cho BS
FAU - Kim, M J
AU  - Kim MJ
FAU - Cha, D R
AU  - Cha DR
FAU - Ha, H
AU  - Ha H
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Nephron
JT  - Nephron
JID - 0331777
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Naphthalenes)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - I271P23G24 (calphostin C)
RN  - IY9XDZ35W2 (Glucose)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Diabetic Neuropathies/physiopathology
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - Glomerular Mesangium/*metabolism
MH  - Glucose/*pharmacology
MH  - Humans
MH  - Monocytes/*metabolism
MH  - Naphthalenes/pharmacology
MH  - Protein Kinase C/antagonists & inhibitors
MH  - RNA, Messenger/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1998/06/03 02:04
MHDA- 2000/10/06 11:01
CRDT- 1998/06/03 02:04
PHST- 1998/06/03 02:04 [pubmed]
PHST- 2000/10/06 11:01 [medline]
PHST- 1998/06/03 02:04 [entrez]
AID - nef79033 [pii]
AID - 10.1159/000044988 [doi]
PST - ppublish
SO  - Nephron. 1998;79(1):33-7. doi: 10.1159/000044988.