PMID- 9609524
OWN - NLM
STAT- MEDLINE
DCOM- 19980618
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 101
IP  - 2
DP  - 1998 May
TI  - Interleukin-6 is expressed by plasma cells from patients with multiple myeloma 
      and monoclonal gammopathy of undetermined significance.
PG  - 287-95
AB  - Interleukin-6 (IL-6) is an important growth factor for human myeloma cells in 
      vitro and in vivo. However, the identity of the cells producing IL-6 in vivo in 
      patients with multiple myeloma (MM) remains the subject of debate. We have 
      developed a sensitive dual-colour fluorescence in situ hybridization (FISH) 
      technique to investigate the expression of IL-6 mRNA by individual bone marrow 
      plasma cells from patients with multiple myeloma, monoclonal gammopathy of 
      undetermined significance (MGUS) and healthy subjects. IL-6 mRNA could be 
      identified in all immunoglobulin light chain (IgLC) expressing cells from all 
      patients with MM and MGUS. The IL-6 protein could also be detected by direct 
      immunofluorescence in all plasma cells (cytoplasmic light chain positive) from 
      all patients with MM and MGUS. Furthermore, it was also possible to demonstrate 
      cytoplasmic IL-6 staining of plasma cells from patients with MM by flow 
      cytometric analysis. In contrast, neither the IL-6 mRNA or protein could be 
      detected in normal plasma cells from healthy bone marrow donors. These data 
      demonstrate that plasma cells from patients with MM and MGUS express the IL-6 
      mRNA and synthesize the IL-6 protein and support the hypothesis that autocrine 
      synthesis of IL-6 is of importance in patients with MM.
FAU - Sati, H I
AU  - Sati HI
AD  - Department of Human Metabolism and Clinical Biochemistry, University of Sheffield 
      Medical School.
FAU - Apperley, J F
AU  - Apperley JF
FAU - Greaves, M
AU  - Greaves M
FAU - Lawry, J
AU  - Lawry J
FAU - Gooding, R
AU  - Gooding R
FAU - Russell, R G
AU  - Russell RG
FAU - Croucher, P I
AU  - Croucher PI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Color
MH  - Female
MH  - Humans
MH  - Immunoglobulin Light Chains/analysis
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interleukin-6/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Monoclonal Gammopathy of Undetermined Significance/*metabolism
MH  - Multiple Myeloma/*metabolism
MH  - Plasma Cells/*metabolism
EDAT- 1998/06/03 00:00
MHDA- 1998/06/03 00:01
CRDT- 1998/06/03 00:00
PHST- 1998/06/03 00:00 [pubmed]
PHST- 1998/06/03 00:01 [medline]
PHST- 1998/06/03 00:00 [entrez]
AID - 10.1046/j.1365-2141.1998.00687.x [doi]
PST - ppublish
SO  - Br J Haematol. 1998 May;101(2):287-95. doi: 10.1046/j.1365-2141.1998.00687.x.