PMID- 9609529
OWN - NLM
STAT- MEDLINE
DCOM- 19980618
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 101
IP  - 2
DP  - 1998 May
TI  - Distinction of eosinophilic leukaemia from idiopathic hypereosinophilic syndrome 
      by analysis of Wilms' tumour gene expression.
PG  - 325-34
AB  - In patients presenting with immature eosinophilic precursors it is notoriously 
      difficult to distinguish acute eosinophilic leukaemia (EoL) from the benign 
      idiopathic hypereosinophilic syndrome (HES), based on morphological, cytochemical 
      and immunophenotyping criteria, alone. Cytogenetic analysis or fluorescence in 
      situ hybridization (FISH) can help in discriminating between these rare 
      haematological disorders, but often treatment decisions cannot wait for the 
      results of these time-consuming techniques. Recently, we and others found Wilms' 
      tumour (WT1) gene expression to be increased in virtually all patients with acute 
      leukaemias, whereas normal haemopoietic progenitors express the WT1 gene at much 
      lower levels or not at all. To determine whether detection of WT1 gene expression 
      is useful to distinguish EoL from HES patients, we analysed, by RT-PCR, bone 
      marrow or blood mononuclear cells from EoL (n=3), HES (n=3) and reactive 
      eosinophilia patients (n = 4) for WT1 gene expression. Using our WT1-RT-PCR 
      protocol, we found WT1 gene expression to be restricted to EoL patients. By 
      detecting WT1 mRNA transcripts in the cerebrospinal fluid using RT-PCR, we were 
      also able to diagnose isolated CNS-relapsed leukaemia, initially confused with 
      bacterial meningitis, in an EoL patient. In conclusion, we show that WT1-RT-PCR 
      is a powerful complementary diagnostic tool to distinguish acute eosinophilic 
      leukaemia from the hypereosinophilic syndromes. This observation needs 
      confirmation in a larger series of EoL and HES patients.
FAU - Menssen, H D
AU  - Menssen HD
AD  - Medizinische Klinik III, Hamatologie, Onkologie und Transfusionsmedizin, 
      Universitatsklinikum Benjamin Franklin der Freien Universitat Berlin, Germany.
FAU - Renkl, H J
AU  - Renkl HJ
FAU - Rieder, H
AU  - Rieder H
FAU - Bartelt, S
AU  - Bartelt S
FAU - Schmidt, A
AU  - Schmidt A
FAU - Notter, M
AU  - Notter M
FAU - Thiel, E
AU  - Thiel E
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Central Nervous System Neoplasms/diagnosis
MH  - Child
MH  - Diagnosis, Differential
MH  - Female
MH  - Gene Expression
MH  - *Genes, Wilms Tumor
MH  - Humans
MH  - Hypereosinophilic Syndrome/*diagnosis/genetics
MH  - Leukemia, Eosinophilic, Acute/*diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
EDAT- 1998/06/03 00:00
MHDA- 1998/06/03 00:01
CRDT- 1998/06/03 00:00
PHST- 1998/06/03 00:00 [pubmed]
PHST- 1998/06/03 00:01 [medline]
PHST- 1998/06/03 00:00 [entrez]
AID - 10.1046/j.1365-2141.1998.00701.x [doi]
PST - ppublish
SO  - Br J Haematol. 1998 May;101(2):325-34. doi: 10.1046/j.1365-2141.1998.00701.x.