PMID- 9609664
OWN - NLM
STAT- MEDLINE
DCOM- 19980729
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 6
IP  - 3
DP  - 1998 Apr
TI  - Molecular cytogenetic analysis of heterochromatin in the chromosomes of tilapia, 
      Oreochromis niloticus (Teleostei: Cichlidae).
PG  - 205-11
AB  - The structure of the heterochromatic bands in mitotic chromosomes of the 
      important tropical aquaculture species of tilapia, Oreochromis niloticus, was 
      investigated by the combination of the C-banding technique, chromosomal digestion 
      with two restriction endonucleases and fluorescence in situ hybridization (FISH) 
      of two satellite DNAs (SATA and SATB). The tilapia chromosomes presented 
      heterochromatic bands in the centromeres and in the short arms of almost all 
      chromosomes that were differentially digested by the restriction endonucleases 
      HaeIII and EcoRI. FISH of SATA showed that this satellite sequence is distributed 
      in the centromeric region of all chromosomes of tilapia. FISH also revealed an 
      intense hybridization signal for SATB in only one chromosome pair, but less 
      intense signals were also present in several other pairs. The digestion of 
      tilapia chromosomes by HaeIII and EcoRI was positively correlated with the 
      position of SATA and SATB in chromosomes as revealed by FISH. The results 
      obtained may be useful in future molecular and genetic studies of tilapias.
FAU - Oliveira, C
AU  - Oliveira C
AD  - Departamento de Morfologia, Instituto de Biociencias, UNESP, Botucatu, Sao Paulo, 
      Brazil.
FAU - Wright, J M
AU  - Wright JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA, Satellite)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Animals
MH  - Chromosome Banding
MH  - Chromosomes/*chemistry
MH  - DNA, Satellite/chemistry
MH  - Female
MH  - Heterochromatin/*genetics
MH  - Human Genome Project
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Restriction Mapping
MH  - Tilapia/*genetics
EDAT- 1998/06/03 00:00
MHDA- 1998/06/03 00:01
CRDT- 1998/06/03 00:00
PHST- 1998/06/03 00:00 [pubmed]
PHST- 1998/06/03 00:01 [medline]
PHST- 1998/06/03 00:00 [entrez]
AID - 10.1023/a:1009211701829 [doi]
PST - ppublish
SO  - Chromosome Res. 1998 Apr;6(3):205-11. doi: 10.1023/a:1009211701829.