PMID- 9610376
OWN - NLM
STAT- MEDLINE
DCOM- 19980615
LR  - 20191210
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 246
IP  - 2
DP  - 1998 May 19
TI  - Cyclic AMP induces ryanodine-sensitive Ca2+ release from microsomal vesicles of 
      rat parotid acinar cells.
PG  - 422-5
AB  - The effect of cAMP on a ryanodine-sensitive Ca2+ release from microsomal vesicles 
      of rat parotid acinar cells was studied. After a steady state of ATP-dependent 
      45Ca2+ uptake into the vesicles, cAMP was added to the medium with thapsigargin 
      (TG) to block a reuptake of 45Ca2+. The addition of cAMP (1.0 mM) with TG 
      released about 10% of the 45Ca2+ that had been taken up. The cAMP-induced 45Ca2+ 
      release was strongly inhibited by pretreatment of the vesicles with 500 microMM 
      ryanodine. Preincubation with cAMP (1 mM) abolished ryanodine (10 microM)-induced 
      45Ca2+ release. The presence of a specific inhibitor of cAMP-dependent protein 
      kinase (PKA) H-89 (10 microMM) inhibited the cAMP-induced 45Ca2+ release. These 
      results indicate that in rat parotid acinar cells, cAMP can activate a 
      ryanodine-sensitive Ca2+ release mechanism in the endoplasmic reticulum and that 
      this activation is via a PKA-dependent process.
FAU - Ozawa, T
AU  - Ozawa T
AD  - Department of Physiology, Tohoku University School of Medicine, Sendai, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Isoquinolines)
RN  - 0 (Sulfonamides)
RN  - 130964-32-8 (H 85)
RN  - 15662-33-6 (Ryanodine)
RN  - 67526-95-8 (Thapsigargin)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9005-49-6 (Heparin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - M876330O56 (N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/antagonists & inhibitors
MH  - Cyclic AMP/*pharmacology
MH  - Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors
MH  - Enzyme Inhibitors/pharmacology
MH  - Heparin/pharmacology
MH  - In Vitro Techniques
MH  - Ion Transport/drug effects
MH  - Isoquinolines/pharmacology
MH  - Male
MH  - Microsomes/drug effects/metabolism
MH  - Parotid Gland/cytology/*drug effects/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Ryanodine/*pharmacology
MH  - *Sulfonamides
MH  - Thapsigargin/pharmacology
EDAT- 1998/06/04 00:00
MHDA- 1998/06/04 00:01
CRDT- 1998/06/04 00:00
PHST- 1998/06/04 00:00 [pubmed]
PHST- 1998/06/04 00:01 [medline]
PHST- 1998/06/04 00:00 [entrez]
AID - S0006-291X(98)98636-3 [pii]
AID - 10.1006/bbrc.1998.8636 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1998 May 19;246(2):422-5. doi: 
      10.1006/bbrc.1998.8636.