PMID- 9611071
OWN - NLM
STAT- MEDLINE
DCOM- 19980709
LR  - 20190816
IS  - 0009-9163 (Print)
IS  - 0009-9163 (Linking)
VI  - 53
IP  - 2
DP  - 1998 Feb
TI  - Maternal cell contamination of buccal smear samples in nursing neonates.
PG  - 114-8
AB  - Buccal smear analysis is a non-invasive method which is being popularized by new 
      fluorescence in situ hybridization (FISH) techniques. It is frequently used for 
      gender identification and detection of sex chromosome aneuploidy in neonates. We 
      attempted to determine whether or not buccal smears of nursing infants can be 
      contaminated by maternal cells from breast feeding. FISH involving centromere 
      specific directly labeled, multicolor probes for chromosomes X, Y and 18 were 
      used for analysis of buccal smear samples. Buccal smear samples from 22 breast 
      fed and 20 formula fed male neonates were analyzed in a blinded fashion. 
      Twenty-seven percent of samples from breast fed infants had some (0.5-2.5%) XX 
      signal pattern while the samples from formula fed infants had no XX signal 
      pattern (difference statistically significant, p < 0.02, at 95% confidence 
      interval). Our results indicate that breast feeding can cause maternal cell 
      contamination of buccal smear samples that can lead to misinterpretation of 
      results involving FISH analysis or other DNA based diagnostic studies. We have 
      also modified the FISH technique to suit the neonates.
FAU - Babovic-Vuksanovic, D
AU  - Babovic-Vuksanovic D
AD  - Department of Medical Genetics, Mayo Clinic and Mayo Foundation, Rochester, 
      Minnesota 55905, USA.
FAU - Michels, V V
AU  - Michels VV
FAU - Law, M E
AU  - Law ME
FAU - Lindor, N M
AU  - Lindor NM
FAU - Jalal, S M
AU  - Jalal SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Clin Genet
JT  - Clinical genetics
JID - 0253664
SB  - IM
MH  - *Breast Feeding
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant, Newborn
MH  - Male
MH  - Mouth Mucosa/*cytology
MH  - Prospective Studies
MH  - *Sex Determination Processes
EDAT- 1998/06/04 00:00
MHDA- 1998/06/04 00:01
CRDT- 1998/06/04 00:00
PHST- 1998/06/04 00:00 [pubmed]
PHST- 1998/06/04 00:01 [medline]
PHST- 1998/06/04 00:00 [entrez]
AID - 10.1111/j.1399-0004.1998.tb02657.x [doi]
PST - ppublish
SO  - Clin Genet. 1998 Feb;53(2):114-8. doi: 10.1111/j.1399-0004.1998.tb02657.x.