PMID- 9611099
OWN - NLM
STAT- MEDLINE
DCOM- 19980611
LR  - 20071114
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 149
IP  - 6
DP  - 1998 Jun
TI  - Persistence of radiation-induced translocations in human peripheral blood 
      determined by chromosome painting.
PG  - 602-13
AB  - We have investigated the persistence of translocations and other types of 
      chromosome damage with time using human peripheral blood acutely exposed in vitro 
      to 137Cs gamma rays at doses ranging from 0.5 to 4 Gy. Freshly drawn blood from 
      one donor was irradiated and metaphase chromosomes were prepared 2 to 7 days 
      after exposure. Chromosomes 1, 2 and 4 were painted red-orange and chromosomes 3, 
      5 and 6 were painted green by fluorescence in situ hybridization (FISH) using 
      "semi-directly" labeled whole-chromosome painting probes. This type of labeling 
      combines direct and indirect labeling and showed significant advantages over both 
      these other methods. All types of structural chromosome aberrations were 
      classified by the Protocol for Aberration Identification and Nomenclature 
      Terminology (PAINT) system. The yields of dicentric chromosomes, acentric 
      fragments and ring chromosomes diminished with time as expected. Translocations 
      exhibited greater persistence but showed a clear and statistically significant 
      reduction in frequency at all doses. The mathematical model suggested that the 
      translocation frequencies would reach a plateau of approximately 4, 15, 51, 106 
      and 179 translocations per 100 cell equivalents after irradiation with 0.5, 1, 2, 
      3 and 4 Gy, respectively. When translocations were classified by the conventional 
      system, an analysis of the distribution of translocations and dicentrics per cell 
      indicated that both types of exchanges were Poisson-distributed 48 h 
      postirradiation. However, cells bearing translocations have a higher possibility 
      of having dicentrics than cells without translocations. These findings suggest 
      that dicentrics may contribute to a decline of translocation frequencies with 
      time, and that some translocations are not completely persistent. The results 
      obtained here using human blood exposed in vitro may influence the use of 
      translocations as a retrospective biodosimeter of exposure to ionizing radiation 
      in humans.
FAU - Matsumoto, K
AU  - Matsumoto K
AD  - Biology and Biotechnology Research Program, Lawrence Livermore National 
      Laboratory, Livermore, California 94551, USA.
FAU - Ramsey, M J
AU  - Ramsey MJ
FAU - Nelson, D O
AU  - Nelson DO
FAU - Tucker, J D
AU  - Tucker JD
LA  - eng
GR  - P01 CA59431/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Cells, Cultured
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*radiation effects/ultrastructure
MH  - Male
MH  - *Translocation, Genetic
EDAT- 1998/06/04 00:00
MHDA- 1998/06/04 00:01
CRDT- 1998/06/04 00:00
PHST- 1998/06/04 00:00 [pubmed]
PHST- 1998/06/04 00:01 [medline]
PHST- 1998/06/04 00:00 [entrez]
PST - ppublish
SO  - Radiat Res. 1998 Jun;149(6):602-13.