PMID- 9612262
OWN - NLM
STAT- MEDLINE
DCOM- 19980701
LR  - 20190329
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 274
IP  - 5
DP  - 1998 May
TI  - Involvement of the 5-HT3 receptor in CRH-induce defecation in rats.
PG  - G827-31
LID - 10.1152/ajpgi.1998.274.5.G827 [doi]
AB  - We evaluated the possibility that serotonin (5-HT) mediates defecation induced by 
      corticotropin-releasing hormone (CRH) exogenously administered or released from 
      the central nervous system by stress via the 5-HT3 receptor in rats. 
      Intracerebroventricular (i.c.v.) injection of CRH (1, 3, and 10 micrograms/rat) 
      dose dependently increased the number of stools excreted in rats, whereas 
      intravenous (i.v.) injection of up to 100 micrograms/kg CRH did not affect 
      defecation. alpha-Helical CRH-(9-41) and 5-HT3 receptor antagonists ramosetron 
      and azasetron inhibited CRH (10 micrograms i.c.v.)-induced defecation in a 
      dose-dependent manner with ED50 values of 4.3 micrograms/kg i.v., 3.8 
      micrograms/kg p.o., and 70.4 micrograms/kg p.o., respectively. alpha-Helical 
      CRH-(9-41) also inhibited CRH-induced defecation by i.c.v. injection with an ED50 
      value of 0.078 microgram/rat. In contrast, ramosetron and azasetron injected 
      i.c.v. had no effect on CRH-induced defecation. alpha-Helical CRH-(9-41), 
      ramosetron, and azasetron reduced defecation caused by restraint stress with ED50 
      values of 0.32, 3.6, and 19.7 micrograms/kg i.v., respectively. These results 
      indicate that CRH exogenously administered or released from the central nervous 
      system by stress peripherally promotes the release of 5-HT, which in turn 
      stimulates defecation through the 5-HT3 receptor.
FAU - Miyata, K
AU  - Miyata K
AD  - Pharmacology Laboratories, Institute for Drug Discovery Research, Ibaraki, Japan.
FAU - Ito, H
AU  - Ito H
FAU - Fukudo, S
AU  - Fukudo S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Benzimidazoles)
RN  - 0 (Bridged Bicyclo Compounds, Heterocyclic)
RN  - 0 (Hormone Antagonists)
RN  - 0 (Oxazines)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Serotonin)
RN  - 0 (Serotonin Antagonists)
RN  - 77HC7URR9Z (azasetron)
RN  - 7ZRO0SC54Y (ramosetron)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - 96118-75-1 (corticotropin releasing hormone (9-41))
SB  - IM
MH  - Animals
MH  - Benzimidazoles/pharmacology
MH  - Bridged Bicyclo Compounds, Heterocyclic/pharmacology
MH  - Corticotropin-Releasing Hormone/*pharmacology
MH  - Defecation/drug effects/*physiology
MH  - Hormone Antagonists/pharmacology
MH  - Injections, Intraventricular
MH  - Male
MH  - Oxazines/pharmacology
MH  - Peptide Fragments/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Serotonin/*physiology
MH  - Restraint, Physical
MH  - Serotonin Antagonists/pharmacology
MH  - Stress, Physiological/physiopathology
EDAT- 1998/06/05 00:00
MHDA- 1998/06/05 00:01
CRDT- 1998/06/05 00:00
PHST- 1998/06/05 00:00 [pubmed]
PHST- 1998/06/05 00:01 [medline]
PHST- 1998/06/05 00:00 [entrez]
AID - 10.1152/ajpgi.1998.274.5.G827 [doi]
PST - ppublish
SO  - Am J Physiol. 1998 May;274(5):G827-31. doi: 10.1152/ajpgi.1998.274.5.G827.