PMID- 9614183
OWN - NLM
STAT- MEDLINE
DCOM- 19980710
LR  - 20240214
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 9
IP  - 6
DP  - 1998 Jun
TI  - Collagenase-3 induction in rat lung fibroblasts requires the combined effects of 
      tumor necrosis factor-alpha and 12-lipoxygenase metabolites: a model of 
      macrophage-induced, fibroblast-driven extracellular matrix remodeling during 
      inflammatory lung injury.
PG  - 1411-24
AB  - The mechanisms responsible for the induction of matrix-degrading proteases during 
      lung injury are ill defined. Macrophage-derived mediators are believed to play a 
      role in regulating synthesis and turnover of extracellular matrix at sites of 
      inflammation. We find a localized increase in the expression of the rat 
      interstitial collagenase (MMP-13; collagenase-3) gene from fibroblastic cells 
      directly adjacent to macrophages within silicotic rat lung granulomas. 
      Conditioned medium from macrophages isolated from silicotic rat lungs was found 
      to induce rat lung fibroblast interstitial collagenase gene expression. 
      Conditioned medium from primary rat lung macrophages or J774 monocytic cells 
      activated by particulates in vitro also induced interstitial collagenase gene 
      expression. Tumor necrosis factor-alpha (TNF-alpha) alone did not induce 
      interstitial collagenase expression in rat lung fibroblasts but did in rat skin 
      fibroblasts, revealing tissue specificity in the regulation of this gene. The 
      activity of the conditioned medium was found to be dependent on the combined 
      effects of TNF-alpha and 12-lipoxygenase-derived arachidonic acid metabolites. 
      The fibroblast response to this conditioned medium was dependent on de novo 
      protein synthesis and involved the induction of nuclear activator protein-1 
      activity. These data reveal a novel requirement for macrophage-derived 
      12-lipoxygenase metabolites in lung fibroblast MMP induction and provide a 
      mechanism for the induction of resident cell MMP gene expression during 
      inflammatory lung processes.
FAU - Mariani, T J
AU  - Mariani TJ
AD  - Department of Internal Medicine, Washington University School of Medicine at 
      Barnes-Jewish Hospital, St. Louis, Missouri 63110, USA. mariani@biochem.wustl.edu
FAU - Sandefur, S
AU  - Sandefur S
FAU - Roby, J D
AU  - Roby JD
FAU - Pierce, R A
AU  - Pierce RA
LA  - eng
GR  - P01 HL029594/HL/NHLBI NIH HHS/United States
GR  - R29 HL054049/HL/NHLBI NIH HHS/United States
GR  - F32 HL009179/HL/NHLBI NIH HHS/United States
GR  - HL-09179/HL/NHLBI NIH HHS/United States
GR  - HL-54049/HL/NHLBI NIH HHS/United States
GR  - R01 HL054049/HL/NHLBI NIH HHS/United States
GR  - HL-29594/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - EC 1.13.11.31 (Arachidonate 12-Lipoxygenase)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.- (Mmp13 protein, mouse)
RN  - EC 3.4.24.- (Mmp13 protein, rat)
SB  - IM
MH  - Animals
MH  - Arachidonate 12-Lipoxygenase/*metabolism
MH  - Arachidonic Acid/metabolism
MH  - Cell Line
MH  - Collagenases/*biosynthesis/genetics
MH  - Culture Media, Conditioned
MH  - Enzyme Induction
MH  - Extracellular Matrix/metabolism/physiology
MH  - Fibroblasts/*enzymology
MH  - Gene Expression
MH  - Kinetics
MH  - Lung/immunology
MH  - Lung Diseases/enzymology
MH  - Lung Injury
MH  - Macrophages/*metabolism
MH  - Matrix Metalloproteinase 13
MH  - Mice
MH  - Models, Biological
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Silicosis/enzymology
MH  - Transcription Factor AP-1/metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
PMC - PMC25362
EDAT- 1998/06/17 00:00
MHDA- 1998/06/17 00:01
CRDT- 1998/06/17 00:00
PHST- 1998/06/17 00:00 [pubmed]
PHST- 1998/06/17 00:01 [medline]
PHST- 1998/06/17 00:00 [entrez]
AID - 0613 [pii]
AID - 10.1091/mbc.9.6.1411 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1998 Jun;9(6):1411-24. doi: 10.1091/mbc.9.6.1411.