PMID- 9614909
OWN - NLM
STAT- MEDLINE
DCOM- 19980618
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 103
IP  - 2
DP  - 1998 Jun
TI  - Detection of RB1 deletions by fluorescence in situ hybridization in malignant 
      hematologic disorders.
PG  - 117-23
AB  - We evaluated the usefulness of fluorescence in situ hybridization (FISH) using 
      different-colored commercial RB1 and 13qter DNA probes to identify RB1 deletions 
      in interphase nuclei of bone marrow from 24 patients with agnogenic myeloid 
      metaplasia (AMM), 20 patients with multiple myeloma (MM), 21 patients with other 
      hematologic malignancies, and 25 normal bone marrow transplant (BMT) donors. 
      Based on the 25 normal BMT donors, the upper boundary for the normal percentage 
      of nuclei with one RB1 signal was 6.5%. Based on eight specimens known to have a 
      deletion of 13q14 by cytogenetic studies, the lower limit of abnormal for the 
      percentage of nuclei with one RB1 signal was 12.5%. More than 12.5% of nuclei had 
      a single RB1 signal in 7/24 (29%) patients with AMM and 3/20 (15%) patients with 
      MM. None of the 21 patients with hematologic malignancies other than AMM or MM 
      had more than 12.5% nuclei with loss of RB1. The results of this study suggest 
      that FISH with RB1 probes is useful to detect loss of RB1 in interphase nuclei 
      from patients with hematologic disorders who have chromosome abnormalities 
      involving 13q14. Thus, FISH with probes for RB1 is efficacious to investigate the 
      pathogenesis of RB1 in malignant neoplasms and is a useful adjunct to 
      conventional cytogenetic studies in clinical practice when abnormalities of 13q14 
      are involved.
FAU - Juneau, A L
AU  - Juneau AL
AD  - Division of Laboratory Genetics, Mayo Clinic, Rochester, MN 55905, USA.
FAU - Kaehler, M
AU  - Kaehler M
FAU - Christensen, E R
AU  - Christensen ER
FAU - Schad, C R
AU  - Schad CR
FAU - Zinsmeister, A R
AU  - Zinsmeister AR
FAU - Lust, J
AU  - Lust J
FAU - Hanson, C
AU  - Hanson C
FAU - Dewald, G W
AU  - Dewald GW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Retinoblastoma Protein)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow Transplantation
MH  - Chromosomes, Human, Pair 13/genetics
MH  - Female
MH  - *Gene Deletion
MH  - Hematologic Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics
MH  - Primary Myelofibrosis/*genetics
MH  - Retinoblastoma Protein/*genetics
EDAT- 1998/06/06 00:00
MHDA- 1998/06/06 00:01
CRDT- 1998/06/06 00:00
PHST- 1998/06/06 00:00 [pubmed]
PHST- 1998/06/06 00:01 [medline]
PHST- 1998/06/06 00:00 [entrez]
AID - S0165460897004081 [pii]
AID - 10.1016/s0165-4608(97)00408-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Jun;103(2):117-23. doi: 
      10.1016/s0165-4608(97)00408-1.