PMID- 9614916
OWN - NLM
STAT- MEDLINE
DCOM- 19980618
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 103
IP  - 2
DP  - 1998 Jun
TI  - E1A transformed normal human prostate epithelial cells contain a 16q deletion.
PG  - 155-63
AB  - The difficulty of maintaining long-term prostate cell cultures has hindered the 
      development of essential models for understanding prostate cancer. We report here 
      the establishment of two 12S E1A transformed non-tumorigenic prostate epithelial 
      cell strains, and their characterization. The two clonal cell strains, TP2 and 
      TP4, proliferated for approximately 40 passages before senescence. Both exhibited 
      a strong dependence on exogenous peptide growth factors and an immunophenotype 
      characteristic of their prostate epithelial origin. Cytogenetic analysis revealed 
      a consistent deletion on the q arm of chromosome 16 in TP2 with an otherwise 
      normal karyotype. Band-specific microdissection generated region-specific probes 
      from 16q23, which when used in fluorescence in situ hybridization (FISH) revealed 
      that the region was deleted in 83% of metaphases analyzed. By cytogenetic 
      analysis and FISH, the q arm of 16 was found deleted from the genome of TP4 in 
      60% of cells analyzed. Lost sequences on 16q-16q23 in particular--in prostate 
      cancer have been observed by a variety of methods. Localization of common region 
      of deletion has been determined from these studies to be distal to 16q23. Our 
      findings suggest that 16q23 may be of major importance in the development of 
      prostate cancer, and may harbor tumor suppressor elements.
FAU - Chin, R K
AU  - Chin RK
AD  - Johns Hopkins School of Medicine, Department of Pathology, Baltimore, MD, USA.
FAU - Hawkins, A L
AU  - Hawkins AL
FAU - Isaacs, W B
AU  - Isaacs WB
FAU - Griffin, C A
AU  - Griffin CA
LA  - eng
GR  - 2P30CA 06973-29/CA/NCI NIH HHS/United States
GR  - CA 53286/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Adenovirus E1A Proteins)
SB  - IM
MH  - Adenovirus E1A Proteins/genetics
MH  - Animals
MH  - Cell Culture Techniques/methods
MH  - Cell Line, Transformed
MH  - Cell Transformation, Viral/*genetics
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 16/*genetics
MH  - Epithelial Cells/*cytology/*metabolism/virology
MH  - Humans
MH  - Karyotyping
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Middle Aged
MH  - Prostate/*cytology/*metabolism
EDAT- 1998/06/06 00:00
MHDA- 1998/06/06 00:01
CRDT- 1998/06/06 00:00
PHST- 1998/06/06 00:00 [pubmed]
PHST- 1998/06/06 00:01 [medline]
PHST- 1998/06/06 00:00 [entrez]
AID - S016546089700407X [pii]
AID - 10.1016/s0165-4608(97)00407-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Jun;103(2):155-63. doi: 
      10.1016/s0165-4608(97)00407-x.