PMID- 9618023
OWN - NLM
STAT- MEDLINE
DCOM- 19980626
LR  - 20190831
IS  - 0960-0760 (Print)
IS  - 0960-0760 (Linking)
VI  - 64
IP  - 5-6
DP  - 1998 Mar
TI  - Oestradiol up-regulates oestrogen receptor, cyclophilin, and glyceraldehyde 
      phosphate dehydrogenase mRNA concentrations in endometrium, but down-regulates 
      them in liver.
PG  - 231-7
AB  - Oestradiol regulates reproductive physiology and cardiovascular health in women. 
      In the endometrium of ovariectomized ewes, previous work demonstrated that a 
      single dose of oestradiol (50 microg) up-regulates oestrogen receptor-alpha (ER) 
      and progesterone receptor (PR) gene expression within 24 h. Here we compared 
      responses to different doses of oestradiol and different dosing regimens in two 
      diverse tissues: endometrium and liver. ER, c-fos, cyclophilin and glyceraldehyde 
      phosphate dehydrogenase (GAPDH) mRNA concentrations were analyzed on replicate 
      RNA slot blots in both tissues, while PR and apolipoprotein AI (apo AI) mRNA 
      concentrations were only analyzed in endometrium or liver, respectively. Along 
      with ER mRNA, oestradiol strongly up-regulated GAPDH and cyclophilin mRNA 
      concentrations in endometrium. In liver, however, oestradiol down-regulated them, 
      along with apo AI mRNA. Responses to different doses and dose regimens, including 
      repeated 50 microg doses, were similar to those evoked by a single 50 microg dose 
      of oestradiol. Thus, oestradiol appears to have all-or-none effects which include 
      up-regulation of ER, cyclophilin and GAPDH gene expression in endometrium and 
      down-regulation of ER, apo AI, cyclophilin and GAPDH gene expression in liver. 
      These results illustrate the sharp contrast between two mammalian tissues in 
      their responses to physiological levels of oestradiol.
FAU - Zou, K
AU  - Zou K
AD  - Department of Animal Science, Institute of Biosciences and Technology, Texas A&M 
      University, College Station 77843-2471, USA. ning@cvm.tamu.edu
FAU - Ing, N H
AU  - Ing NH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0 (Apolipoprotein A-I)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 5.2.1.8 (Peptidylprolyl Isomerase)
SB  - IM
MH  - Animals
MH  - Apolipoprotein A-I/genetics
MH  - Down-Regulation/physiology
MH  - Endometrium/*drug effects
MH  - Estradiol/*pharmacology
MH  - Estrogen Receptor alpha
MH  - Female
MH  - Gene Expression Regulation/*drug effects
MH  - Genes, fos/genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*genetics
MH  - Liver/*drug effects
MH  - Ovariectomy
MH  - Peptidylprolyl Isomerase/*genetics
MH  - RNA, Messenger/*metabolism
MH  - Receptors, Estrogen/*genetics
MH  - Receptors, Progesterone/genetics
MH  - Sheep
MH  - Up-Regulation/physiology
EDAT- 1998/06/09 00:00
MHDA- 1998/06/09 00:01
CRDT- 1998/06/09 00:00
PHST- 1998/06/09 00:00 [pubmed]
PHST- 1998/06/09 00:01 [medline]
PHST- 1998/06/09 00:00 [entrez]
AID - S0960-0760(97)00194-5 [pii]
AID - 10.1016/s0960-0760(97)00194-5 [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 1998 Mar;64(5-6):231-7. doi: 
      10.1016/s0960-0760(97)00194-5.