PMID- 9618890
OWN - NLM
STAT- MEDLINE
DCOM- 19980806
LR  - 20191210
IS  - 0165-7380 (Print)
IS  - 0165-7380 (Linking)
VI  - 22
IP  - 3
DP  - 1998 Apr
TI  - A comparison of the activities of certain enzymes related to energy metabolism in 
      leukocytes in dogs and cats.
PG  - 187-92
AB  - The activities of Na+,K(+)-ATPase in plasma membrane, of cytosolic enzymes and of 
      glutamate dehydrogenase (GlGD) in mitochondria were measured in leukocytes (WBC) 
      from dogs and cats to clarify the differences in energy metabolism in these 
      cells. Feline WBC had significantly higher activities of hexokinase (HK), 
      pyruvate kinase (PK) and LDH with pyruvate as substrate than did canine WBC. 
      Canine WBC had significantly higher activities of glucokinase (GK) and GlDH than 
      did feline WBC. Feline WBC had unique characteristics of energy metabolism in 
      that the activities of the cytosolic enzymes under anaerobic conditions were 
      significantly higher than those in canine WBC. It therefore appears that there 
      are distinct differences in glucose-metabolism in WBC between dogs and cats. WBC 
      enzyme activities are considered to reflect the metabolic state in the whole body 
      of the animal. It is therefore suggested that changes in the activities of 
      certain glycolytic enzymes in WBC may be useful as a diagnostic indicator in some 
      types of metabolic disease in dogs and cats.
FAU - Washizu, T
AU  - Washizu T
AD  - Division of Clinical Pathology, Nippon Veterinary and Animal Science University, 
      Tokyo, Japan.
FAU - Kuramoto, E
AU  - Kuramoto E
FAU - Abe, M
AU  - Abe M
FAU - Sako, T
AU  - Sako T
FAU - Arai, T
AU  - Arai T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Vet Res Commun
JT  - Veterinary research communications
JID - 8100520
RN  - 0 (Blood Glucose)
RN  - 0 (Enzymes)
RN  - 0 (Insulin)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 1.4.1.2 (Glutamate Dehydrogenase)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - EC 2.7.1.- (Fructokinases)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.2 (Glucokinase)
RN  - EC 2.7.1.4 (fructokinase)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Aspartate Aminotransferases/blood
MH  - Blood Glucose/analysis
MH  - Cats/blood/*metabolism
MH  - Cell Membrane/enzymology
MH  - Cytosol/enzymology
MH  - Dogs/blood/*metabolism
MH  - Energy Metabolism
MH  - Enzymes/*blood
MH  - Female
MH  - Fructokinases/blood
MH  - Glucokinase/blood
MH  - Glucosephosphate Dehydrogenase/blood
MH  - Glutamate Dehydrogenase/blood
MH  - Hexokinase/blood
MH  - Insulin/blood
MH  - L-Lactate Dehydrogenase/blood
MH  - Leukocytes/*enzymology/ultrastructure
MH  - Male
MH  - Mitochondria/enzymology
MH  - Pyruvate Kinase/blood
MH  - Reference Values
MH  - Sodium-Potassium-Exchanging ATPase/blood
EDAT- 1998/06/10 00:00
MHDA- 1998/06/10 00:01
CRDT- 1998/06/10 00:00
PHST- 1998/06/10 00:00 [pubmed]
PHST- 1998/06/10 00:01 [medline]
PHST- 1998/06/10 00:00 [entrez]
AID - 10.1023/a:1006073305479 [doi]
PST - ppublish
SO  - Vet Res Commun. 1998 Apr;22(3):187-92. doi: 10.1023/a:1006073305479.