PMID- 9619430
OWN - NLM
STAT- MEDLINE
DCOM- 19980812
LR  - 20190909
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 43
IP  - 2
DP  - 1998 Summer
TI  - Effects of polyethylene particles on tissue surrounding knee arthroplasties in 
      rabbits.
PG  - 123-30
AB  - Clinical studies suggest a role for polyethylene (PE) wear debris in the 
      pathogenesis of osteolysis and loosening of total joint replacements. In this 
      study, submicron particles of ultrahigh molecular weight PE (UHMWPE) were placed 
      around pressfit tibial hemiarthroplasties in rabbits to determine the biological 
      reaction. After 6 months the periprosthetic tissue was harvested and 
      characterized biochemically by measuring the extracellular matrix macromolecules, 
      collagen, and glycosaminoglycan (GAG) and quantifying the expression of 
      inflammatory/osteolytic mediators [prostaglandin E2 (PGE2), hexosaminidase, 
      transforming growth factor beta (TGF beta), and interleukins-6 and -1 (IL-6, 
      IL-1)]. Particle exposure resulted in a decrease in levels of total extracellular 
      matrix molecules including a 53% decrease in total GAG (p < 0.05) and a 74% 
      decrease in total collagen (p < 0.005). Collagen content remained significantly 
      decreased when normalized for cellularity (DNA content). Total TGF beta release 
      exhibited a downward trend (p = 0.06) in the particle exposed group. 
      Hexosaminidase and PGE2 levels did not show a difference between groups; however, 
      when normalized for cellularity, PGE2 values exhibited an upward trend in the 
      particle exposed group (p = 0.1). IL-6 was undetected by bioassay and ELISA. 
      Previous studies emphasized that PE debris enhances the degradation of bone. The 
      data from this in vivo model suggest that submicron UHMWPE particles may also act 
      to inhibit biosynthetic pathways of bone and mesenchymal tissue. Decreased levels 
      of collagen, GAG, and TGF beta expression may indicate suppression of bone 
      formation, possibly through a downregulation of osteoblast activity.
FAU - Sacomen, D
AU  - Sacomen D
AD  - Division of Orthopaedic Surgery, Stanford University School of Medicine, 
      California, USA.
FAU - Smith, R L
AU  - Smith RL
FAU - Song, Y
AU  - Song Y
FAU - Fornasier, V
AU  - Fornasier V
FAU - Goodman, S B
AU  - Goodman SB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Polyethylenes)
RN  - EC 3.4.24.- (Collagenases)
SB  - IM
MH  - Animals
MH  - *Arthroplasty, Replacement, Knee
MH  - Collagenases/metabolism
MH  - Cytokines/biosynthesis
MH  - Extracellular Matrix/enzymology/metabolism
MH  - Foreign-Body Reaction/enzymology/metabolism/*pathology
MH  - Inflammation Mediators/metabolism
MH  - Male
MH  - Polyethylenes/*adverse effects
MH  - Rabbits
EDAT- 1998/06/10 02:03
MHDA- 2000/06/20 09:00
CRDT- 1998/06/10 02:03
PHST- 1998/06/10 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/06/10 02:03 [entrez]
AID - 10.1002/(SICI)1097-4636(199822)43:2<123::AID-JBM6>3.0.CO;2-Q [pii]
AID - 10.1002/(sici)1097-4636(199822)43:2<123::aid-jbm6>3.0.co;2-q [doi]
PST - ppublish
SO  - J Biomed Mater Res. 1998 Summer;43(2):123-30. doi: 
      10.1002/(sici)1097-4636(199822)43:2<123::aid-jbm6>3.0.co;2-q.