PMID- 9620204
OWN - NLM
STAT- MEDLINE
DCOM- 19980810
LR  - 20191210
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 53
IP  - 2
DP  - 1998 Feb
TI  - Cellular membrane factors are the major determinants of porcine reproductive and 
      respiratory syndrome virus tropism.
PG  - 121-8
AB  - The mechanism that restricts porcine reproductive and respiratory syndrome virus 
      (PRRSV) replication in a variety of cell-lines has been investigated in this 
      study. Using a virus binding assay, it was found that PRRSV could not bind to 
      most cell-lines tested. However, vero cells, which are non-permissive to PRRSV 
      infection, were able to bind and internalize a virus almost as efficiently as the 
      permissive cell-line MARC-145. In addition, MARC-145 and vero cells internalized 
      PRRSV in an infectious form, indicating that virus entry occurred by receptor 
      mediated endocytosis. Polyethylene glycol (PEG)-mediated fusion of virus with 
      cells induced the production of infectious virus from vero and MARC-145, but not 
      from the others cells tested. Infectious virus was also recovered from vero and 
      several other non-permissive cell types after transfection of viral RNA, 
      indicating that the viral genome is infectious per se. Thus, absence of PRRSV 
      binding to cells might be one major determinant of PRRSV cell tropism. However, 
      because vero cells restricted PRRSV replication following virus binding and 
      internalization but prior to RNA replication, it is possible that multiple viral 
      and cellular components might be involved in allowing PRRSV replication on cells.
FAU - Kreutz, L C
AU  - Kreutz LC
AD  - Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA 50010, USA. lckreutz@creta.ccr.ufsm.br
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
SB  - IM
MH  - Animals
MH  - Cats
MH  - Cattle
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cricetinae
MH  - Dogs
MH  - Membrane Fusion
MH  - Porcine respiratory and reproductive syndrome virus/*metabolism
MH  - Swine
MH  - Vero Cells
EDAT- 1998/06/10 00:00
MHDA- 1998/06/10 00:01
CRDT- 1998/06/10 00:00
PHST- 1998/06/10 00:00 [pubmed]
PHST- 1998/06/10 00:01 [medline]
PHST- 1998/06/10 00:00 [entrez]
AID - S0168170297001342 [pii]
AID - 10.1016/s0168-1702(97)00134-2 [doi]
PST - ppublish
SO  - Virus Res. 1998 Feb;53(2):121-8. doi: 10.1016/s0168-1702(97)00134-2.