PMID- 9620358
OWN - NLM
STAT- MEDLINE
DCOM- 19980805
LR  - 20071114
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 18
IP  - 5
DP  - 1998 May
TI  - Differential expression of p53 tumor suppressor protein and IL-2 in activated T 
      cells from elderly humans.
PG  - 315-20
AB  - Aging is associated with a decline in T cell proliferative responses and 
      aberrations in cytokine production. In the present study, we examined if aging 
      might alter the expression of the tumor-suppressor protein p53 and the 
      retinoblastoma susceptibility gene product (Rb) as well as the levels of Bcl-2 in 
      resting and activated human T cells. No significant differences were observed in 
      the basal levels of p53 protein among resting T cells from young and elderly 
      humans. After stimulation with anti-CD3 monoclonal antibody (mAb) OKT3 and 
      phorbol myristate acetate (PMA), T cells from young humans exhibited severalfold 
      increases in p53 protein expression compared with resting T cells. By contrast, T 
      cells from a substantial portion of elderly humans failed to demonstrate 
      significant increases in p53 in response to anti-CD3 plus PMA. No age-related 
      alterations in the levels of Rb or Bcl-2 proteins were observed in resting or 
      anti-CD3/PMA-stimulated T cells. To delineate whether the age-related reductions 
      in p53 expression might be linked to decreased interleukin-2 (IL-2) production, 
      we compared the expression of p53 and IL-2 in anti-CD3/PMA-stimulated T cells 
      from elderly people. The results showed that impaired induction of p53 expression 
      in activated T cells from certain elderly people could be observed without 
      considerable impairments in IL-2 production. These observations suggest that 
      age-related reductions in T cell expression of p53 may contribute to the decline 
      of T cell competence independent of the impairments in IL-2 production.
FAU - VanAman, S E
AU  - VanAman SE
AD  - Department of Internal Medicine, The William H. Davis Medical Research Center, 
      The Ohio State University, Columbus 43210, USA.
FAU - Whisler, R L
AU  - Whisler RL
LA  - eng
GR  - A03763/PHS HHS/United States
PT  - Clinical Trial
PT  - Comparative Study
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Interleukin-2)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Basal Metabolism
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - Interleukin-2/*biosynthesis
MH  - *Lymphocyte Activation
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-bcl-2/biosynthesis
MH  - Statistics, Nonparametric
MH  - T-Lymphocytes/*metabolism
MH  - Tumor Suppressor Protein p53/*biosynthesis
EDAT- 1998/06/10 00:00
MHDA- 1998/06/10 00:01
CRDT- 1998/06/10 00:00
PHST- 1998/06/10 00:00 [pubmed]
PHST- 1998/06/10 00:01 [medline]
PHST- 1998/06/10 00:00 [entrez]
AID - 10.1089/jir.1998.18.315 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 1998 May;18(5):315-20. doi: 10.1089/jir.1998.18.315.