PMID- 9620446
OWN - NLM
STAT- MEDLINE
DCOM- 19980807
LR  - 20061115
IS  - 0145-5680 (Print)
IS  - 0145-5680 (Linking)
VI  - 44
IP  - 3
DP  - 1998 May
TI  - The human breast cancer cell line IIB-BR-G has amplified c-myc and c-fos 
      oncogenes in vitro and is spontaneously metastatic in vivo.
PG  - 493-504
AB  - IIB-BR-G is an undifferentiated, highly heterogeneous, hormone receptor negative 
      human breast cancer cell line previously established in our laboratory from a 
      patient's primary tumor. An in vitro growing cell line (IIB-BR-G) and a 
      xenotransplanted tumor growing in nude mice (IIB-BR-G(NUDE)) were derived. To 
      further characterize these systems, immunocytochemical analysis was performed for 
      differentiation antigens (PEM 200 kDa, CEA, NCA 90 kDa), blood-group related 
      antigens (Le(x), sTn), oncogenes and tumor suppressor gene products (Her-2/neu 
      protein, p53), metastasis-related cathepsin D and CD63/5.01 Ag, and the chemokine 
      monocyte chemotactic protein 1 (MCP-1). Expression of markers was heterogeneous 
      in these different systems. Previously reported karyotypic analysis has shown 
      extensive chromosomal alterations including double min. Searching for oncogene 
      amplification, we detected augmented copy number of c-myc and c-fos, the last one 
      with two rearranged fragments. No amplification was found for c-erbB-2 in the 
      cell line or in IIB-BR-G(NUDE), although this oncogene was amplified in the 
      patient's primary tumor DNA. The differences observed between the patient's 
      tumor, the cell line and the IIB-BR-G(NUDE) tumors are probably due to clonal 
      expansion of cell variants not present in the original tumor. Electron microscopy 
      of IIB-BR-G growing cells revealed epithelial characteristics with abundant dense 
      granules, presumably secretory, distributed all over the cytoplasm and great 
      nuclear pleomorphism. In vitro, IIB-BR-G cells showed a significant number of 
      invading cells by Matrigel assay. After nearly 40 sequential subcutaneous 
      passages of the original xenograft through nude mice, 80% of recipients developed 
      spontaneous metastases, primarily to the lung and lymph nodes. Since this 
      experimental model allowed to analyze changes produced in cancer cells from the 
      primary tumor during adaptation to in vitro and in vivo growth, our results 
      provide novel insights on the behaviour of hormone independent metastatic breast 
      cancer.
FAU - Bover, L
AU  - Bover L
AD  - Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias 
      Exactas y Naturales, Universidad de Buenos Aires, Argentina.
FAU - Barrio, M
AU  - Barrio M
FAU - Bravo, A I
AU  - Bravo AI
FAU - Slavutsky, I
AU  - Slavutsky I
FAU - Larripa, I
AU  - Larripa I
FAU - Bolondi, A
AU  - Bolondi A
FAU - Ayala, M
AU  - Ayala M
FAU - Mordoh, J
AU  - Mordoh J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Receptors, Estrogen)
SB  - IM
MH  - Animals
MH  - Antigens, Neoplasm/biosynthesis
MH  - Breast Neoplasms
MH  - Carcinoma, Ductal, Breast
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Invasiveness
MH  - Proto-Oncogene Proteins c-fos/*genetics
MH  - Proto-Oncogene Proteins c-myc/*genetics
MH  - Receptors, Estrogen/genetics
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured
EDAT- 1998/06/10 00:00
MHDA- 1998/06/10 00:01
CRDT- 1998/06/10 00:00
PHST- 1998/06/10 00:00 [pubmed]
PHST- 1998/06/10 00:01 [medline]
PHST- 1998/06/10 00:00 [entrez]
PST - ppublish
SO  - Cell Mol Biol (Noisy-le-grand). 1998 May;44(3):493-504.