PMID- 9621125
OWN - NLM
STAT- MEDLINE
DCOM- 19980723
LR  - 20061115
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 15
IP  - 3
DP  - 1998 Jun 10
TI  - [Chromosome assignment of a novel microsatellite polymorphic marker (D14S1435) by 
      fluorescence in situ hybridization].
PG  - 161-3
AB  - OBJECTIVE: Chromosome assignment of a novel microsatellite marker (D14S1435) 
      containing CA repeats and showing 11 alleles and 0.85 of PIC value in Chinese 
      population) isolated from microdissected human chromosome 14q24.3 DNA library by 
      fluorescence in situ hybridization(FISH). METHODS: The polymorphic marker was 
      used as probe to screen a human Lambda/DASH genome library, a positive clone was 
      isolated and the recombinant phage DNA was extracted as primary material. The 
      insert was recovered through BamH I digestion to complete and low melting point 
      agarose gel electro- phoresis, after processed with Sau3A I the probe was 
      prepared by linker- catch PCR method and the following fluorescence in situ 
      hybridization(FISH) was conducted to metaphase chromosomes. RESULTS: this novel 
      STR was precisely mapped to human chromosome 14q24.3, and this demonstrated the 
      validity of isolating chromosome band-specific genetic markers from probe pool 
      made by chromosome microdissection. CONCLUSION: Confirmed by chromosome 
      fluorescence in situ hybridization, the novel STR obtained by chromosome 
      microdissection, PCR and subcloning method was really from the micro- dissected 
      region. This not only increases the numbers of genetic markers used for linkage 
      analysis in the region,but also provides a novel valuable genetic marker for the 
      gene diagnosis and gene cloning of the possible disease-causing genes located in 
      the region of 14q24.3.
FAU - Zheng, Q
AU  - Zheng Q
AD  - State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan 
      University, Shanghai, 200433 P.R.China.
FAU - Yu, L
AU  - Yu L
FAU - Zhang, M
AU  - Zhang M
FAU - Hu, P
AU  - Hu P
FAU - Mao, N
AU  - Mao N
FAU - Xu, Y
AU  - Xu Y
FAU - Zhao, S
AU  - Zhao S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Chromosome Mapping
MH  - *Genetic Markers
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Microsatellite Repeats
EDAT- 1998/06/11 00:00
MHDA- 1998/06/11 00:01
CRDT- 1998/06/11 00:00
PHST- 1998/06/11 00:00 [pubmed]
PHST- 1998/06/11 00:01 [medline]
PHST- 1998/06/11 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1998 Jun 10;15(3):161-3.