PMID- 9621985
OWN - NLM
STAT- MEDLINE
DCOM- 19980807
LR  - 20151119
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 43
IP  - 6
DP  - 1998 Jun
TI  - Monocyte chemoattractant protein-1 and interleukin-8 levels in urine and serum of 
      patents with hemolytic uremic syndrome.
PG  - 759-67
AB  - The epidemic form of the hemolytic uremic syndrome (HUS) in children is 
      hallmarked by endothelial cell damage, most predominantly displayed by the 
      glomerular capillaries. The influx of mononuclear (MO) and polymorphonuclear 
      cells (PMNs) into the glomeruli may be an important event in the initiation, 
      prolongation, and progression of glomerular endothelial cell damage in HUS 
      patients. The molecular mechanisms for the recruitment of these leukocytes into 
      the kidney are unclear, but monocyte chemoattractant protein-1 (MCP-1) and IL-8 
      are suggested to be prime candidates. In this study, we analyzed the presence of 
      both chemokines in 24-h urinary (n = 15) and serum (n = 14) samples of HUS 
      children by specific ELISAs. Furthermore, kidney biopsies of three different HUS 
      children were examined for MO and PMN cell infiltration by histochemical 
      techniques and electron microscopy. Whereas the chemokines MCP-1 and IL-8 were 
      present in only very limited amounts in urine of 17 normal control subjects, 
      serial samples of HUS patients demonstrated significantly elevated levels of both 
      chemokines. HUS children with anuria showed higher initial and maximum chemokine 
      levels than their counterparts without anuria. A strong positive correlation was 
      observed between urinary MCP-1 and IL-8 levels. Whereas initial serum IL-8 levels 
      were significantly increased in HUS children, serum MCP-1 levels were only 
      slightly elevated compared with serum MCP-1 in control children. No correlation 
      was found between urinary and serum chemokine concentrations. Histologic and EM 
      studies of HUS biopsy specimens clearly showed the presence of MOs and to a 
      lesser extent of PMNs in the glomeruli. The present data suggest an important 
      local role for MOs and PMNs in the process of glomerular endothelial-cell damage. 
      The chemokines MCP-1 and IL-8 may possibly be implicated in the pathogenesis of 
      HUS through the recruitment and activation of MOs and PMNs, respectively.
FAU - van Setten, P A
AU  - van Setten PA
AD  - Department of Pediatrics, University Hospital, Nijmegen, The Netherlands.
FAU - van Hinsbergh, V W
AU  - van Hinsbergh VW
FAU - van den Heuvel, L P
AU  - van den Heuvel LP
FAU - Preyers, F
AU  - Preyers F
FAU - Dijkman, H B
AU  - Dijkman HB
FAU - Assmann, K J
AU  - Assmann KJ
FAU - van der Velden, T J
AU  - van der Velden TJ
FAU - Monnens, L A
AU  - Monnens LA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Biomarkers/blood/urine
MH  - Biopsy
MH  - Blood Cell Count
MH  - Chemokine CCL2/*blood/urine
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Hemolytic-Uremic Syndrome/*blood/pathology/urine
MH  - Humans
MH  - Infant
MH  - Interleukin-8/*blood/urine
MH  - Kidney/*pathology/physiopathology
MH  - Male
MH  - Monocytes/pathology
MH  - Neutrophils/pathology
MH  - Reference Values
EDAT- 1998/06/11 00:00
MHDA- 1998/06/11 00:01
CRDT- 1998/06/11 00:00
PHST- 1998/06/11 00:00 [pubmed]
PHST- 1998/06/11 00:01 [medline]
PHST- 1998/06/11 00:00 [entrez]
AID - 10.1203/00006450-199806000-00008 [doi]
PST - ppublish
SO  - Pediatr Res. 1998 Jun;43(6):759-67. doi: 10.1203/00006450-199806000-00008.