PMID- 9623592
OWN - NLM
STAT- MEDLINE
DCOM- 19980827
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 58
IP  - 6
DP  - 1998 Jun
TI  - Differential expression pattern of retinoid X receptors in adult murine 
      testicular cells implies varying roles for these receptors in spermatogenesis.
PG  - 1351-6
AB  - Retinoids have previously been shown to be crucial for normal spermatogenesis. 
      The role of retinoic acid receptors has been studied, but relatively little is 
      known about the function of retinoid X receptors (RXRs). To gain more insight in 
      the function of RXRs during spermatogenesis, the cellular localization of RXRs in 
      the mouse testis was examined using immunohistochemistry and RNase protection 
      assays. In both normal and vitamin A-deficient (VAD) testes, a strong immune 
      response to an RXRalpha antibody occurred in Leydig cells, peritubular myoid 
      cells, and A spermatogonia. Weaker signals were found in spermatocytes and 
      spermatids. In normal testes, an RXRbeta antibody gave a reaction in Leydig 
      cells, and, to a lesser extent, in Sertoli cells, A spermatogonia, pachytene 
      spermatocytes, and spermatids. In Leydig cells, a cytoplasmatic signal was found 
      in addition to the nuclear signal. In the VAD testis, only Leydig cells and A 
      spermatogonia were positive, which indicates that RXRbeta expression may be 
      dependent on the retinoid status. Previous studies have shown RXRgamma mRNA 
      expression in the mouse testis at a low level. Nevertheless, an RXRgamma antibody 
      caused a strong immune response in interstitial cells and in A spermatogonia, and 
      a weak signal in pachytene spermatocytes. These immunohistochemical data were 
      supported by the results of RNase protection assays on mRNA of testicular cell 
      isolations. In conclusion, the different RXRs in the mouse testis have distinct 
      expression patterns, suggesting that they may have different functions.
FAU - Gaemers, I C
AU  - Gaemers IC
AD  - Department of Cell Biology, Utrecht University, The Netherlands. gaemers@nki.nl
FAU - van Pelt, A M
AU  - van Pelt AM
FAU - van der Saag, P T
AU  - van der Saag PT
FAU - Hoogerbrugge, J W
AU  - Hoogerbrugge JW
FAU - Themmen, A P
AU  - Themmen AP
FAU - de Rooij, D G
AU  - de Rooij DG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - *Gene Expression
MH  - Immunohistochemistry
MH  - Leydig Cells/chemistry
MH  - Male
MH  - Mice
MH  - RNA, Messenger/analysis
MH  - Receptors, Retinoic Acid/analysis/*genetics/*physiology
MH  - Retinoid X Receptors
MH  - Ribonucleases
MH  - Sertoli Cells/chemistry
MH  - *Spermatogenesis
MH  - Spermatozoa/chemistry
MH  - Testis/chemistry/*metabolism
MH  - Transcription Factors/analysis/*genetics/*physiology
EDAT- 1998/06/12 00:00
MHDA- 1998/06/12 00:01
CRDT- 1998/06/12 00:00
PHST- 1998/06/12 00:00 [pubmed]
PHST- 1998/06/12 00:01 [medline]
PHST- 1998/06/12 00:00 [entrez]
AID - 10.1095/biolreprod58.6.1351 [doi]
PST - ppublish
SO  - Biol Reprod. 1998 Jun;58(6):1351-6. doi: 10.1095/biolreprod58.6.1351.