PMID- 9625786 OWN - NLM STAT- MEDLINE DCOM- 19980727 LR - 20191024 IS - 0343-8651 (Print) IS - 0343-8651 (Linking) VI - 37 IP - 1 DP - 1998 Jul TI - Incidence of non-01 Vibrio cholerae and Aeromonas spp. in fresh water in Araraquara, Brazil. PG - 28-31 AB - The occurrence of Aeromonas spp., Vibrio cholerae, and Plesiomonas shigelloides in fresh water from various sources in Araraquara, State of Sao Paulo, Brazil was determined. Samples from ten distinct irrigation systems used in vegetable cultivation, from five distinct streams, from two reservoirs, from one artificial lake, and from three distinct springs were analyzed. All isolates were serotyped and tested for hemolysin, cytotoxin, heat-stable (ST) and heat-labile (LT) enterotoxins production; presence of plasmid; autoagglutination and drug resistance. V. cholerae isolates were also tested for cholera enterotoxin (CT) production, and Aeromonas isolates for suicide phenomenon. No P. shigelloides was found. V. cholerae non 01 was found in five irrigation water samples and in three stream samples. Aeromonas sp. were isolated in two samples of irrigation water, in three streams, and in one reservoir. All the V. cholerae and Aeromonas isolates were positive for beta-hemolysin production, and all Aeromonas isolates were positive for suicide phenomenon; cytotoxic activities were observed in two Aeromonas strains. Cholera enterotoxin was not found in eight V. cholerae non-01 isolates tested by the Y-1 mouse adrenal cell. All isolates were also negative for the other virulence markers. V. cholerae isolates were found to be sensitive to the majority of drugs tested, while Aeromonas strains presented multiple drug resistance. FAU - Pasetto Falcao, D AU - Pasetto Falcao D AD - Departamento de Ciencias Biologicas, Faculdade de Ciencias Farmaceuticas, UNESP, Rodovia Araraquara-Jau, Km 1, 14801-902 Araraquara, SP Brazil. FAU - Rogerio Lustri, W AU - Rogerio Lustri W FAU - Maria Bauab, T AU - Maria Bauab T LA - eng PT - Journal Article PL - United States TA - Curr Microbiol JT - Current microbiology JID - 7808448 SB - IM MH - Aeromonas/*isolation & purification MH - Brazil MH - Fresh Water/microbiology MH - Plesiomonas/isolation & purification MH - Vibrio cholerae/*isolation & purification MH - *Water Microbiology EDAT- 1998/06/26 00:00 MHDA- 1998/06/26 00:01 CRDT- 1998/06/26 00:00 PHST- 1998/06/26 00:00 [pubmed] PHST- 1998/06/26 00:01 [medline] PHST- 1998/06/26 00:00 [entrez] AID - 10.1007/s002849900332 [doi] PST - ppublish SO - Curr Microbiol. 1998 Jul;37(1):28-31. doi: 10.1007/s002849900332.