PMID- 9626161
OWN - NLM
STAT- MEDLINE
DCOM- 19980702
LR  - 20171116
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 83
IP  - 6
DP  - 1998 Jun
TI  - Increased CD69 and human leukocyte antigen-DR expression on T lymphocytes in 
      insulin-dependent diabetes mellitus of long standing.
PG  - 2204-9
AB  - To better define prevailing activation of circulating T cell subsets in 
      insulin-dependent diabetes mellitus (IDDM) of recent onset (DM; n = 31; median 
      age +/- SD, 28 +/- 6.9 yr) and of long standing (DML; n = 27; age, 33 +/- 10.4 
      yr; median duration of disease, 105 months), CD4+ and CD8+ T cells were analyzed 
      to determine their naive and memory subsets as well as their expression of human 
      leukocyte antigen (HLA)-DR, interleukin-2 receptor alpha-chain (CD25), and CD69 
      by three-color flow cytometry. Twenty-six healthy subjects (HS; age, 32.0 +/- 8.2 
      yr) served as controls. No deviation was seen in either IDDM group compared to HS 
      in CD25 expression on CD4+ or CD8+ cells or in their CD45RA+ or CD45RA- subsets. 
      HLA-DR expression, however, was increased (P < 0.05) in total CD8+ cells and 
      CD45RA+ cells, with CD45RA- CD8+ cells joining the prevailing pattern only in 
      DML. Among CD4+ cells, increased expression of HLA-DR molecules was restricted to 
      total and CD45RA- cells in DML. CD69 expression did not differ between IDDM and 
      HS, but differed between DML (CD4+, CD8+, and CD45RA- CD4+) and DM only. In 
      conclusion, our data demonstrate that HLA-DR expression in IDDM is restricted to 
      memory cells (CD45RA-) among CD4+ cells in DML and is more markedly confined to 
      naive (CD45RA+) than to memory CD8+ cells, whereas the early activation antigen 
      CD69 is more readily expressed in DML than in DM. The observed activation of 
      circulating T cells suggests an ongoing immune process in IDDM both at clinical 
      manifestation and after long duration.
FAU - Gessl, A
AU  - Gessl A
AD  - Department of Medicine III, University of Vienna, Austria. 
      alois.gessl@akh-wien.ac.at
FAU - Waldhausl, W
AU  - Waldhausl W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD69 antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Interleukin-2)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 1)
SB  - IM
MH  - Adult
MH  - Antigens, CD/*metabolism
MH  - Antigens, Differentiation, T-Lymphocyte/*metabolism
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Diabetes Mellitus, Type 1/*immunology
MH  - Female
MH  - Flow Cytometry
MH  - HLA-DR Antigens/*metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - Lectins, C-Type
MH  - Leukocyte Common Antigens/analysis
MH  - Male
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 1
MH  - Receptors, Interleukin-2/analysis
MH  - T-Lymphocytes/*immunology
MH  - Time Factors
EDAT- 1998/06/17 00:00
MHDA- 1998/06/17 00:01
CRDT- 1998/06/17 00:00
PHST- 1998/06/17 00:00 [pubmed]
PHST- 1998/06/17 00:01 [medline]
PHST- 1998/06/17 00:00 [entrez]
AID - 10.1210/jcem.83.6.4889 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1998 Jun;83(6):2204-9. doi: 10.1210/jcem.83.6.4889.