PMID- 9626354
OWN - NLM
STAT- MEDLINE
DCOM- 19980625
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 76
IP  - 6
DP  - 1998 Jun 10
TI  - Relationship of stable integration of herpes simplex virus-2 Bg/II N subfragment 
      Xho2 to malignant transformation of human papillomavirus-immortalized cervical 
      keratinocytes.
PG  - 865-71
AB  - Transfection of the right end Xho2 subfragment of Bg/II N of herpes simplex 
      virus-2 (HSV-2) into human genital keratinocytes immortalized by human 
      papillomavirus (HPV) type 16 or 18 resulted in invasive and noninvasive indolent 
      cystic squamous carcinomas when cells were injected into immunocompromised mice. 
      Retention and expression of the right end portion of the Bg/II N fragment 
      correlated with malignancy, as the corresponding HSV-2 sequences were integrated 
      and transcribed in the tumorigenic cell lines. HPV-immortalized cells alone were 
      not tumorigenic. In contrast, previous results have shown that using the entire 
      Bg/II N region can malignantly transform HPV-immortalized cells, although HSV2 
      DNA was not retained. Together, these observations localize the transforming 
      activity of Bg/II N to Xho2 and suggest that the remaining sequences have an 
      inhibitory effect on stable integration. The Xho2 sequence is 2480 bp long and 
      contains an open reading frame (ORF) extending from nucleotides 559 to 1797. The 
      ORF encodes a putative protein of 412-aa with a m.w. of 42-43 kDa and is highly 
      homologous to UL43 of HSV-I. The correlation of tumorigenicity with stable 
      integration and expression of Xho2 DNA in HPV-immortalized cells indicates that 
      HSV-2 should be investigated further for a possible role in cervical cancer.
FAU - DiPaolo, J A
AU  - DiPaolo JA
AD  - Division of Basic Sciences, National Cancer Institute, National Institutes of 
      Health, Bethesda 20892, USA. dipaoloj@37a.nci.nih.gov
FAU - Woodworth, C D
AU  - Woodworth CD
FAU - Coutlee, F
AU  - Coutlee F
FAU - Zimonic, D B
AU  - Zimonic DB
FAU - Bryant, J
AU  - Bryant J
FAU - Kessous, A
AU  - Kessous A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *Cell Transformation, Neoplastic
MH  - Cells, Cultured
MH  - DNA, Viral/analysis
MH  - Female
MH  - Herpesvirus 2, Human/*genetics
MH  - Humans
MH  - Keratinocytes/*virology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Papillomaviridae/*genetics
MH  - Uterine Cervical Neoplasms/*etiology
MH  - *Virus Integration
EDAT- 1998/06/17 02:03
MHDA- 2000/06/20 09:00
CRDT- 1998/06/17 02:03
PHST- 1998/06/17 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/06/17 02:03 [entrez]
AID - 10.1002/(SICI)1097-0215(19980610)76:6<865::AID-IJC16>3.0.CO;2-1 [pii]
AID - 10.1002/(sici)1097-0215(19980610)76:6<865::aid-ijc16>3.0.co;2-1 [doi]
PST - ppublish
SO  - Int J Cancer. 1998 Jun 10;76(6):865-71. doi: 
      10.1002/(sici)1097-0215(19980610)76:6<865::aid-ijc16>3.0.co;2-1.