PMID- 9626793
OWN - NLM
STAT- MEDLINE
DCOM- 19980702
LR  - 20190905
IS  - 0277-3732 (Print)
IS  - 0277-3732 (Linking)
VI  - 21
IP  - 3
DP  - 1998 Jun
TI  - Detection of trisomy 7 with fluorescence in situ hybridization and its 
      correlation with DNA content and proliferating cell nuclear antigen-positivity in 
      prostate cancer.
PG  - 253-7
AB  - The authors applied fluorescence in situ hybridization (FISH), using specific DNA 
      probes for chromosome 7, to routine paraffin-embedded tissue sections obtained 
      from 35 radical-prostatectomy specimens. Proliferative activity was also 
      evaluated using static cytometry to assess DNA content and immunohistochemistry 
      for proliferating cell nuclear antigen (PCNA) positivity. These results were 
      correlated with each other and with the morphologic parameters. The presence of 
      three or more of chromosome 7 was observed in 71% of the cases, as was a high S 
      phase, with a triploid prevalent DNA content and a PCNA index above mean value in 
      66% of the cases. No correlation was detected between these findings and 
      histologic grade; conversely, there was a significant correlation with stage 
      (chi-square = 5.33; p = 0.021). From these results, the authors maintain that the 
      presence of an extra chromosome 7-correlated in most cases with an increase in 
      cell kinetics and an advanced stage-may be an additional prognostic marker of 
      aggressive behavior.
FAU - Matturri, L
AU  - Matturri L
AD  - Institute of Pathology, University of Milan, IRCCS, Ospendale Maggiore, Italy.
FAU - Biondo, B
AU  - Biondo B
FAU - Cazzullo, A
AU  - Cazzullo A
FAU - Montanari, E
AU  - Montanari E
FAU - Radice, F
AU  - Radice F
FAU - Timossi, R
AU  - Timossi R
FAU - Turconi, P
AU  - Turconi P
FAU - Lavezzi, A M
AU  - Lavezzi AM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Am J Clin Oncol
JT  - American journal of clinical oncology
JID - 8207754
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Proliferating Cell Nuclear Antigen)
SB  - IM
MH  - Adenocarcinoma/classification/*genetics/pathology/surgery
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - DNA, Neoplasm/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Proliferating Cell Nuclear Antigen/*analysis
MH  - Prostatectomy
MH  - Prostatic Neoplasms/classification/*genetics/pathology/surgery
MH  - *Trisomy
EDAT- 1998/06/17 00:00
MHDA- 1998/06/17 00:01
CRDT- 1998/06/17 00:00
PHST- 1998/06/17 00:00 [pubmed]
PHST- 1998/06/17 00:01 [medline]
PHST- 1998/06/17 00:00 [entrez]
AID - 10.1097/00000421-199806000-00010 [doi]
PST - ppublish
SO  - Am J Clin Oncol. 1998 Jun;21(3):253-7. doi: 10.1097/00000421-199806000-00010.