PMID- 9627012
OWN - NLM
STAT- MEDLINE
DCOM- 19980707
LR  - 20111117
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 41
IP  - 6
DP  - 1998 Jun
TI  - Human anticardiolipin monoclonal autoantibodies cause placental necrosis and 
      fetal loss in BALB/c mice.
PG  - 1026-39
AB  - OBJECTIVE: To analyze the structure, specificity, and in vivo pathogenetic 
      potential of 2 human anticardiolipin (aCL) monoclonal antibodies (MAb). METHODS: 
      Human aCL IgG MAb were generated from hybridized Epstein-Barr virus-induced B 
      cell lines from a healthy subject (MAb 519) and from a patient with primary 
      antiphospholipid syndrome (MAb 516). Studies of antigen-binding specificity and 
      analysis of Ig V-gene mutations were carried out. The MAb were independently 
      injected into mated female BALB/c mice, and their effect on pregnancy outcome was 
      compared with that of MAb 57, a highly mutated and antigen-selected human 
      IgG1lambda rabies virus antibody. RESULTS: Both MAb 519 and MAb 516 utilized 
      minimally mutated V(H)DJ(H) and VkappaJkappa gene segments and bound cardiolipin 
      and other anionic phospholipids in the absence of beta2-glycoprotein I 
      (beta2-GPI). The mice injected with aCL MAb displayed a significantly higher rate 
      of fetal resorption and a significant reduction in fetal and placental weight as 
      compared with those injected with MAb 57. These findings were accompanied by a 
      finding of placental human IgG deposition and necrosis in the aCL MAb-treated 
      animals. CONCLUSION: The results of this study indicate that human aCL IgG that 
      are beta2-GPI independent can induce pathology.
FAU - Ikematsu, W
AU  - Ikematsu W
AD  - Cornell University Medical College, New York, New York, USA.
FAU - Luan, F L
AU  - Luan FL
FAU - La Rosa, L
AU  - La Rosa L
FAU - Beltrami, B
AU  - Beltrami B
FAU - Nicoletti, F
AU  - Nicoletti F
FAU - Buyon, J P
AU  - Buyon JP
FAU - Meroni, P L
AU  - Meroni PL
FAU - Balestrieri, G
AU  - Balestrieri G
FAU - Casali, P
AU  - Casali P
LA  - eng
GR  - AR-40908/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Antibodies, Anticardiolipin)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantibodies)
RN  - 0 (Cardiolipins)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
SB  - IM
CIN - Arthritis Rheum. 1999 Aug;42(8):1783-4. PMID: 10446886
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Anticardiolipin/*immunology
MH  - Antibodies, Monoclonal/*immunology
MH  - Autoantibodies/*immunology
MH  - Base Sequence
MH  - Binding Sites, Antibody/genetics
MH  - Cardiolipins/immunology
MH  - Epitopes/immunology
MH  - Female
MH  - Fetal Resorption/*immunology
MH  - Humans
MH  - Immunoglobulin G/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Necrosis
MH  - Placenta/*immunology/*pathology
MH  - Pregnancy
EDAT- 1998/06/17 00:00
MHDA- 1998/06/17 00:01
CRDT- 1998/06/17 00:00
PHST- 1998/06/17 00:00 [pubmed]
PHST- 1998/06/17 00:01 [medline]
PHST- 1998/06/17 00:00 [entrez]
AID - 10.1002/1529-0131(199806)41:6<1026::AID-ART9>3.0.CO;2-1 [doi]
PST - ppublish
SO  - Arthritis Rheum. 1998 Jun;41(6):1026-39. doi: 
      10.1002/1529-0131(199806)41:6<1026::AID-ART9>3.0.CO;2-1.