PMID- 9627501
OWN - NLM
STAT- MEDLINE
DCOM- 19980825
LR  - 20061115
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 46
IP  - 5
DP  - 1998 May
TI  - [Identification of aneuploids in uncultured amniotic fluid cells by interphase 
      fluorescence in situ hybridization].
PG  - 486-92
AB  - We report the diagnosis of aneuploids in uncultured amniotic fluid cells by the 
      use of interphase fluorescence in situ hybridization (FISH). About a dozen of 
      cases was studied to detect chromosome aneuploids (trisomies 13, 18 and 21, and 
      monosomy X), especially those in the perinatal period. Results of FISH 
      experiments in each specimen were compared with those of conventional chromosome 
      analysis, and showed no discrepancy between them in most cases. FISH analysis 
      revealed that four 18-trisomies had two different cells, one of which showed two 
      fluorescence signals and the other three signals, the result indicating a 
      maternal cell contamination. Thus, special attention should be paid when FISH 
      analysis is adopted to the diagnosis in uncultured amniotic fluid cells in order 
      to avoid misdiagnosis that may originate in maternal cell contamination.
FAU - Sohda, M
AU  - Sohda M
AD  - Kyushu Medical Science Nagasaki Laboratory, Nagasaki.
FAU - Harada, N
AU  - Harada N
FAU - Nishimura, T
AU  - Nishimura T
FAU - Noda, K
AU  - Noda K
FAU - Abe, K
AU  - Abe K
FAU - Niikawa, N
AU  - Niikawa N
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
SB  - IM
MH  - Amniotic Fluid/*cytology
MH  - *Aneuploidy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Pregnancy
EDAT- 1998/06/17 00:00
MHDA- 1998/06/17 00:01
CRDT- 1998/06/17 00:00
PHST- 1998/06/17 00:00 [pubmed]
PHST- 1998/06/17 00:01 [medline]
PHST- 1998/06/17 00:00 [entrez]
PST - ppublish
SO  - Rinsho Byori. 1998 May;46(5):486-92.