PMID- 9627979
OWN - NLM
STAT- MEDLINE
DCOM- 19980629
LR  - 20190831
IS  - 0379-0738 (Print)
IS  - 0379-0738 (Linking)
VI  - 92
IP  - 2-3
DP  - 1998 Apr 5
TI  - Hair analysis for abused drugs by capillary zone electrophoresis with 
      field-amplified sample stacking.
PG  - 201-11
AB  - The present paper describes the methodological optimisation and validation of a 
      capillary zone electrophoresis method for the determination of morphine, cocaine 
      and 3,4-methylenedioxymethamphetamine (MDMA) in hair, with injection based on 
      field-amplified sample stacking. Diode array UV absorption detection was used to 
      improve analytical selectivity and identification power. Analytical conditions: 
      running buffer 100 mM potassium phosphate adjusted to pH 2.5 with phosphoric 
      acid, applied potential 10 kV, temperature 20 degrees C, injection by 
      electromigration at 10 kV for 10 s, detection by UV absorption at the fixed 
      wavelength of 200 nm or by recording the full spectrum between 190 and 400 nm. 
      Injection conditions: the dried hair extracts were reconstituted with a 
      low-conductivity solvent (0.1 mM formic acid), the injection end of the capillary 
      was dipped in water for 5 s without applying pressure (external rinse step), then 
      a plug of 0.1 mM phosphoric acid was loaded by applying 0.5 psi for 10 s and, 
      finally, the sample was injected electrokinetically at 10 kV for 10 s. Under the 
      described conditions, the limit of detection was 2 ng/ml for MDMA, 8 ng/ml for 
      cocaine and 6 ng/ml for morphine (with a signal-to-noise ratio of 5). The lowest 
      concentration suitable for recording interpretable spectra was about 10-20-times 
      the limit of detection of each analyte. The intraday and day-to-day 
      reproducibility of migration times (n = 6), with internal standardisation, was 
      characterised by R.S.D. values < or = 0.6%; peak area R.S.D.s were better than 
      10% in intraday and than 15% in day-to-day experiments. Analytical linearity was 
      good with R2 better than 0.9990 for all the analytes.
FAU - Tagliaro, F
AU  - Tagliaro F
AD  - Institute of Forensic Medicine, University of Verona, Italy.
FAU - Manetto, G
AU  - Manetto G
FAU - Crivellente, F
AU  - Crivellente F
FAU - Scarcella, D
AU  - Scarcella D
FAU - Marigo, M
AU  - Marigo M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Hallucinogens)
RN  - 0 (Narcotics)
RN  - 76I7G6D29C (Morphine)
RN  - I5Y540LHVR (Cocaine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Cocaine/analysis
MH  - *Electrophoresis, Capillary/methods
MH  - Follow-Up Studies
MH  - Hair/*chemistry
MH  - Hallucinogens/*analysis
MH  - Humans
MH  - Morphine/analysis
MH  - N-Methyl-3,4-methylenedioxyamphetamine/analysis
MH  - Narcotics/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Substance-Related Disorders/*diagnosis/metabolism
EDAT- 1998/06/17 00:00
MHDA- 1998/06/17 00:01
CRDT- 1998/06/17 00:00
PHST- 1998/06/17 00:00 [pubmed]
PHST- 1998/06/17 00:01 [medline]
PHST- 1998/06/17 00:00 [entrez]
AID - S0379-0738(98)00016-4 [pii]
AID - 10.1016/s0379-0738(98)00016-4 [doi]
PST - ppublish
SO  - Forensic Sci Int. 1998 Apr 5;92(2-3):201-11. doi: 10.1016/s0379-0738(98)00016-4.