PMID- 9628225
OWN - NLM
STAT- MEDLINE
DCOM- 19980730
LR  - 20190831
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 71
IP  - 1
DP  - 1998 Mar
TI  - Equine monocyte-derived macrophage cultures and their applications for 
      infectivity and neutralization studies of equine infectious anemia virus.
PG  - 87-104
AB  - Equine infectious anemia virus (EIAV) has been shown to infect cells of 
      monocyte/macrophage lineage. These primary cells are intrinsically difficult to 
      obtain, to purify and to culture in vitro for extended periods of time. As a 
      result, most in vitro studies concerning this lentivirus make use of primary 
      equine fibroblasts or transformed canine or feline cell lines. We describe 
      methods that yield reproducibly pure cultures of equine blood monocytes from 
      peripheral blood mononuclear cells. The in vitro differentiation of these cells 
      into mature equine macrophage was verified using various cytochemical staining 
      methods. The equine monocyte-derived macrophage (MDM) cultures were found to 
      replicate cell-adapted and field strains of EIAV more efficiently than cultures 
      of fully differentiated equine splenic macrophage. Having established 
      reproducible and fully differentiated cultures of equine macrophage, in vitro 
      assays of virus infectivity and serum neutralization were developed using the in 
      vivo target cell of EIAV. These procedures, while developed for the EIAV system, 
      should be equally useful for in vitro cultures of other macrophage-tropic 
      pathogens of horses.
FAU - Raabe, M R
AU  - Raabe MR
AD  - Department of Molecular Genetics and Biochemistry, School of Medicine, University 
      of Pittsburgh, Pennsylvania 15261, USA.
FAU - Issel, C J
AU  - Issel CJ
FAU - Montelaro, R C
AU  - Montelaro RC
LA  - eng
GR  - 2R01 CA49296/CA/NCI NIH HHS/United States
GR  - 5RO1 AI25850/AI/NIAID NIH HHS/United States
GR  - 5T32 AIO7487/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Cell Line
MH  - Female
MH  - Horses
MH  - Infectious Anemia Virus, Equine/pathogenicity/*physiology
MH  - Macrophages/*cytology/physiology/virology
MH  - Monocytes/cytology
MH  - Neutralization Tests/*veterinary
MH  - Reproducibility of Results
MH  - Spleen/cytology
MH  - Time Factors
MH  - *Virus Replication
EDAT- 1998/06/17 00:00
MHDA- 1998/06/17 00:01
CRDT- 1998/06/17 00:00
PHST- 1998/06/17 00:00 [pubmed]
PHST- 1998/06/17 00:01 [medline]
PHST- 1998/06/17 00:00 [entrez]
AID - S0166-0934(97)00204-8 [pii]
AID - 10.1016/s0166-0934(97)00204-8 [doi]
PST - ppublish
SO  - J Virol Methods. 1998 Mar;71(1):87-104. doi: 10.1016/s0166-0934(97)00204-8.