PMID- 9628804
OWN - NLM
STAT- MEDLINE
DCOM- 19980724
LR  - 20231213
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 66
IP  - 5
DP  - 1998 May
TI  - The role of laminin-5 in TGF alpha/EGF-mediated corneal epithelial cell motility.
PG  - 569-79
AB  - Transforming growth factor alpha (TGF alpha) and epidermal growth factor (EGF) 
      stimulate corneal epithelial cell wound closure. However, the role of these 
      growth factors in regulating corneal epithelial cell motility on basement 
      membrane proteins such as laminin has not been elucidated. In the present study 
      we demonstrate that in an in vitro model of corneal wound healing, TGF alpha has 
      no deleterious effects on the deposition of the laminin-5 isoform into the 
      extracellular matrix structure underlying epithelial cells resurfacing bare 
      collagenous stroma. In primary culture, a population of corneal epithelial cells 
      are stimulated by TGF alpha or EGF to become highly motile. These cells are 
      associated with an endogenously secreted, and extracellularly deposited, 'trail' 
      of laminin-5. The laminin-5 trail is specifically associated with motile cells, 
      as non-motile corneal epithelium exhibiting numerous cell-cell contacts does not 
      display a similar laminin-5 localization pattern. In contrast to these 
      observations, a preparation of laminin-5 known to promote cell spreading, 
      adhesion, and formation of hemidesmosomes, when presented exogenously to cultured 
      corneal epithelial cells, does not stimulate motility. However, a commercially 
      available preparation of laminin derived from human placenta which does not 
      contain laminin-5 does significantly promote the migration of TGF alpha- or 
      EGF-stimulated corneal epithelial cells. From these results, it is hypothesized 
      that endogenously secreted laminin-5 functions to promote migration in corneal 
      epithelial cells which have been treated with TGF alpha or EGF. Exogenously 
      presented laminin-5 does not function similarly, but functions to promote corneal 
      epithelial cell adhesion.
FAU - Qin, P
AU  - Qin P
AD  - Department of Anatomy and Cell Biology, Wayne State University School of 
      Medicine, Detroit, MI 48201, USA.
FAU - Kurpakus, M A
AU  - Kurpakus MA
LA  - eng
GR  - EY09890/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cattle
MH  - Cell Adhesion Molecules/*pharmacology
MH  - Cell Movement/*drug effects
MH  - Cells, Cultured
MH  - Epidermal Growth Factor/*pharmacology
MH  - Epithelium, Corneal/cytology/*drug effects
MH  - Microscopy, Fluorescence
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Wound Healing/*drug effects
MH  - Kalinin
EDAT- 1998/06/17 00:00
MHDA- 1998/06/17 00:01
CRDT- 1998/06/17 00:00
PHST- 1998/06/17 00:00 [pubmed]
PHST- 1998/06/17 00:01 [medline]
PHST- 1998/06/17 00:00 [entrez]
AID - S0014-4835(97)90455-3 [pii]
AID - 10.1006/exer.1997.0455 [doi]
PST - ppublish
SO  - Exp Eye Res. 1998 May;66(5):569-79. doi: 10.1006/exer.1997.0455.