PMID- 9630510
OWN - NLM
STAT- MEDLINE
DCOM- 19980722
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 414
IP  - 1-3
DP  - 1998 May 11
TI  - Use of acridine orange in: flow cytometric evaluation of erythropoietic 
      cytotoxicity.
PG  - 49-61
AB  - Cytotoxic insult to bone marrow frequently impairs the proliferating and 
      maturational abilities of erythroid cells. Typically, a ratio of enucleated, 
      immature polychromatic erythrocytes (PCE) to mature normochromatic erythrocytes 
      (NCE) is used to assess cytotoxicity in the micronucleus (MN) assay. The effects 
      of cyclophosphamide (CP) on PCE/NCE ratio in rat bone marrow and spleen were 
      assessed by a newly developed flow cytometric procedure using 
      glutaraldehyde-fixed, acridine orange (AO)-stained cells, and compared to manual 
      scoring of PCE/NCE in Wright stained slides. Comparison of methods showed that 
      manual and flow cytometric determination of PCE were not statistically different. 
      Several other parameters of cytotoxicity could be simultaneously assessed because 
      the method allowed use of unfractionated whole bone marrow/spleen cell samples. 
      Absolute numbers of total nucleated cells (TNC), a ratio of TNC to total 
      erythrocytes (TE), and determination of RNA content within the PCE population 
      demonstrated dose- and time-dependent effects with CP treatment. Shifts in RNA 
      content were particularly sensitive, correctly identifying all CP-treated from 
      control specimens, even in those samples where PCE/NCE ratio was similar. The AO 
      methodology provided a more rapid, statistically-superior, and thorough approach 
      in the assessment of bone marrow and spleen cytotoxicity than the conventional 
      manual method of scoring PCE/NCE ratio alone.
CI  - Copyright 1998 Elsevier Science B.V.
FAU - Criswell, K A
AU  - Criswell KA
AD  - Pathology and Experimental Toxicology, Parke-Davis Pharmaceutical Research, 
      Warner-Lambert, Ann Arbor, MI 48105, USA. criswek@aa.wl.com
FAU - Krishna, G
AU  - Krishna G
FAU - Zielinski, D
AU  - Zielinski D
FAU - Urda, G A
AU  - Urda GA
FAU - Theiss, J C
AU  - Theiss JC
FAU - Juneau, P
AU  - Juneau P
FAU - Bleavins, M R
AU  - Bleavins MR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Alkylating Agents)
RN  - 63231-63-0 (RNA)
RN  - 8N3DW7272P (Cyclophosphamide)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Alkylating Agents/pharmacology
MH  - Animals
MH  - Bone Marrow Cells/physiology
MH  - Cell Death
MH  - Cyclophosphamide/pharmacology
MH  - Erythrocytes/*physiology
MH  - Erythroid Precursor Cells/*physiology
MH  - Flow Cytometry/*methods
MH  - Micronucleus Tests/*methods
MH  - RNA/analysis
MH  - Rats
MH  - Spleen/physiology
EDAT- 1998/06/19 00:00
MHDA- 1998/06/19 00:01
CRDT- 1998/06/19 00:00
PHST- 1998/06/19 00:00 [pubmed]
PHST- 1998/06/19 00:01 [medline]
PHST- 1998/06/19 00:00 [entrez]
AID - S1383-5718(98)00041-2 [pii]
AID - 10.1016/s1383-5718(98)00041-2 [doi]
PST - ppublish
SO  - Mutat Res. 1998 May 11;414(1-3):49-61. doi: 10.1016/s1383-5718(98)00041-2.