PMID- 9630520
OWN - NLM
STAT- MEDLINE
DCOM- 19980722
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 414
IP  - 1-3
DP  - 1998 May 11
TI  - Use of acridine orange in: flow cytometric assessment of micronuclei induction.
PG  - 63-75
AB  - The micronucleus assay is a widely accepted method for evaluation of clastogens 
      and aneugens. In the current study, acridine orange (AO) supravital staining was 
      adapted for flow cytometric usage to assess micronucleated cells in rat bone 
      marrow and spleen. Cyclophosphamide was used as a positive control test compound 
      and results were compared to manual scoring in Wright-stained slides. In bone 
      marrow, both manual and flow cytometric methods demonstrated positive dose 
      response-trends for micronucleated polychromatic erythrocytes (MNPCE). 
      Significant elevations in MNPCE were observed at all doses of cyclophosphamide, 
      and comparisons between methods in bone marrow were not statistically different. 
      The flow cytometric method was more sensitive in spleen samples, showing dose- 
      and time-related increases in micronuclei compared with manual scoring. AO proved 
      to be a sensitive discriminator of RNA and DNA, allowing distinct separation of 
      polychromatic erythrocytes (PCE), normochromic erythrocytes (NCE), total 
      nucleated cells (TNC), and micronucleated populations within both PCE and NCE 
      regions. These results support the use of AO-based flow cytometry to provide a 
      rapid and sensitive indicator of micronuclei inducers.
CI  - Copyright 1998 Elsevier Science B. V.
FAU - Criswell, K A
AU  - Criswell KA
AD  - Pathology and Experimental Toxicology, Parke-Davis Pharmaceutical Research, 
      Warner-Lambert, Ann Arbor, MI 48105, USA. criswek@aa.wl.com
FAU - Krishna, G
AU  - Krishna G
FAU - Zielinski, D
AU  - Zielinski D
FAU - Urda, G A
AU  - Urda GA
FAU - Theiss, J C
AU  - Theiss JC
FAU - Juneau, P
AU  - Juneau P
FAU - Bleavins, M R
AU  - Bleavins MR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 8N3DW7272P (Cyclophosphamide)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Cyclophosphamide/pharmacology
MH  - Evaluation Studies as Topic
MH  - Flow Cytometry/*methods
MH  - Micronucleus Tests/*methods
MH  - Rats
MH  - Spleen/cytology
EDAT- 1998/06/19 00:00
MHDA- 1998/06/19 00:01
CRDT- 1998/06/19 00:00
PHST- 1998/06/19 00:00 [pubmed]
PHST- 1998/06/19 00:01 [medline]
PHST- 1998/06/19 00:00 [entrez]
AID - S1383-5718(98)00042-4 [pii]
AID - 10.1016/s1383-5718(98)00042-4 [doi]
PST - ppublish
SO  - Mutat Res. 1998 May 11;414(1-3):63-75. doi: 10.1016/s1383-5718(98)00042-4.