PMID- 9630611
OWN - NLM
STAT- MEDLINE
DCOM- 19990329
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 793
IP  - 1-2
DP  - 1998 May 18
TI  - Recombinant adeno-associated virus vector: use for transgene expression and 
      anterograde tract tracing in the CNS.
PG  - 169-75
AB  - We used a recombinant adeno-associated virus vector (AAV) to deliver a foreign 
      gene, green fluorescent protein (GFP), into mature neurons in adult rat CNS in 
      vivo. Microinjections of AAV as small as 50 nl transduced hundreds of neurons at 
      the injection site. There was virtually no retrograde transport as fewer than one 
      neuron per brain was found distant from the injection site that exhibited GFP 
      immunoreactivity. The gene product, GFP, filled the entire neuronal cytoplasmic 
      compartment; GFP immunoreactivity was robust in cell bodies, axons, and nerve 
      terminals. There was no tissue damage at the injection sites or pathogenicity 
      indicated by changes in astrocytic or microglial markers. There was no 
      inflammatory response as judged by leukocytic invasion. Gene expression in 
      transduced cells was robust and apparently permanent: there was no evidence of 
      phenotypic reversion up to 12 weeks following infection. AAV is an excellent 
      vector for introducing foreign genes into mature CNS neurons. Not only might it 
      be an ideal vehicle for gene therapy, but also the GFP-containing AAV presents a 
      new strategy for tracing long axonal pathways in the CNS, which is difficult with 
      current tracers (PHAL, biotinylated dextrans).
CI  - Copyright 1998 Elsevier Science B.V.
FAU - Chamberlin, N L
AU  - Chamberlin NL
AD  - Departments of Neurology, Harvard Medical School, Beth Israel Deaconess Medical 
      Center, Boston, MA 02215, USA. nchamber@bidmc.harvard.edu
FAU - Du, B
AU  - Du B
FAU - de Lacalle, S
AU  - de Lacalle S
FAU - Saper, C B
AU  - Saper CB
LA  - eng
GR  - NS22835/NS/NINDS NIH HHS/United States
GR  - R01 NS033987/NS/NINDS NIH HHS/United States
GR  - R01 AG019597/AG/NIA NIH HHS/United States
GR  - R29 AG012401-06/AG/NIA NIH HHS/United States
GR  - AG21856/AG/NIA NIH HHS/United States
GR  - NS33987/NS/NINDS NIH HHS/United States
GR  - R01 AG019597-01/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Luminescent Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Axonal Transport/genetics/*physiology
MH  - Brain/*physiology
MH  - Cell Line
MH  - Dependovirus/*genetics
MH  - *Gene Expression
MH  - Genes, Reporter
MH  - Genetic Vectors/*pharmacology
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins/analysis/genetics/metabolism
MH  - Male
MH  - Neural Pathways/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombination, Genetic
MH  - Transgenes/*genetics
PMC - PMC4961038
MID - NIHMS23534
EDAT- 1998/06/19 00:00
MHDA- 1998/06/19 00:01
PMCR- 2016/07/26
CRDT- 1998/06/19 00:00
PHST- 1998/06/19 00:00 [pubmed]
PHST- 1998/06/19 00:01 [medline]
PHST- 1998/06/19 00:00 [entrez]
PHST- 2016/07/26 00:00 [pmc-release]
AID - S0006-8993(98)00169-3 [pii]
AID - 10.1016/s0006-8993(98)00169-3 [doi]
PST - ppublish
SO  - Brain Res. 1998 May 18;793(1-2):169-75. doi: 10.1016/s0006-8993(98)00169-3.