PMID- 9631042
OWN - NLM
STAT- MEDLINE
DCOM- 19980707
LR  - 20121115
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 14
IP  - 8
DP  - 1996 Aug
TI  - A method to codetect introduced genes and their products in gene therapy 
      protocols.
PG  - 1012-6
AB  - To monitor the presence of introduced genes and the distribution of the encoded 
      proteins in host tissues after gene transfer, we combined fluorescence in situ 
      hybridization (FISH) and immunohistochemistry in two separate gene therapy 
      paradigms. In brain tissue sections from animals injected with pHSVlac vector, we 
      localized nuclei containing vector DNA both in cells expressing and not 
      expressing beta-galactosidase (beta-gal). This suggests that the efficiency of 
      gene transfer is affected not only by gene delivery, but also by cellular 
      controls on gene expression. In a second paradigm, following myoblast 
      transplantation, we detected donor nuclei in the muscle of a patient with 
      Duchenne's muscular dystrophy. The donor nuclei were either surrounded by host 
      nuclei or apparently fused in the patient's muscle fiber producing dystrophin. 
      The combined FISH and immunohistochemistry assay offers greater sensitivity and 
      more information than currently used polymerase chain reaction and protein 
      detection methods.
FAU - Gussoni, E
AU  - Gussoni E
AD  - Division of Genetics, Children's Hospital, Boston, MA 02115, USA.
FAU - Wang, Y
AU  - Wang Y
FAU - Fraefel, C
AU  - Fraefel C
FAU - Miller, R G
AU  - Miller RG
FAU - Blau, H M
AU  - Blau HM
FAU - Geller, A I
AU  - Geller AI
FAU - Kunkel, L M
AU  - Kunkel LM
LA  - eng
GR  - AG10827/AG/NIA NIH HHS/United States
GR  - NS23740/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
RN  - 0 (Dystrophin)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Brain/*metabolism
MH  - *Clinical Protocols
MH  - Dystrophin/genetics
MH  - Female
MH  - *Genetic Therapy
MH  - Herpesvirus 1, Human/genetics/isolation & purification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Muscle, Skeletal/*metabolism
MH  - Muscular Dystrophies/genetics/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1996/08/01 00:00
MHDA- 1998/06/19 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1998/06/19 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 10.1038/nbt0896-1012 [doi]
PST - ppublish
SO  - Nat Biotechnol. 1996 Aug;14(8):1012-6. doi: 10.1038/nbt0896-1012.