PMID- 9633928
OWN - NLM
STAT- MEDLINE
DCOM- 19980708
LR  - 20220414
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 18
IP  - 6
DP  - 1998 Jun
TI  - Cyclic stretch upregulates production of interleukin-8 and monocyte chemotactic 
      and activating factor/monocyte chemoattractant protein-1 in human endothelial 
      cells.
PG  - 894-901
AB  - In vivo, vascular walls are exposed to mechanical stretch, which may promote 
      atherogenesis. This study was designed to investigate the effect of mechanical 
      stretch on the production and gene expression of cytokines in endothelial cells 
      (ECs) of human umbilical veins. ECs were cultured on flexible silicone membranes 
      and exposed to cyclic mechanical stretch. Although the secretion levels of 
      interleukin (IL)-1beta, tumor necrosis factor-alpha, IL-6, granulocyte (G) 
      -colony stimulating factor (CSF), G and macrophage (M) -CSF, and M-CSF were not 
      affected by cyclic stretch over 24 hours, the levels of IL-8 and monocyte 
      chemotactic and activating factor (MCAF)/monocyte chemoattractant protein-1 
      (MCP-1) were significantly increased by cyclic stretch. Northern blot analysis 
      indicated that the mRNA levels of IL-8 and MCAF/MCP-1 were upregulated by cyclic 
      stretch as a function of its intensity. Cytochalasin D, which disrupts the actin 
      cytoskeleton, abolished the stretch-induced gene expression of IL-8 and 
      MCAF/MCP-1. In contrast, neither inhibition of stretch-activated ion channels nor 
      disruption of microtubules affected the induction of these chemokines by cyclic 
      stretch. Northern blot analysis using enzyme inhibitors showed that phospholipase 
      C, protein kinase C, and tyrosine kinase were involved in the stretch-induced 
      gene expression of IL-8 and MCAF/MCP-1, whereas cAMP- or cGMP-dependent protein 
      kinase was not. In conclusion, cyclic stretch enhanced the secretion and gene 
      expression of IL-8 and MCAF/MCP-1 in a stretch-dependent fashion, and the 
      integrity of the actin cytoskeleton and activities of phospholipase C, protein 
      kinase C, and tyrosine kinase may be essential in the process of stretch-induced 
      gene induction of IL-8 and MCAF/MCP-1.
FAU - Okada, M
AU  - Okada M
AD  - Department of Cardiovascular Medicine, Kyoto University, Japan.
FAU - Matsumori, A
AU  - Matsumori A
FAU - Ono, K
AU  - Ono K
FAU - Furukawa, Y
AU  - Furukawa Y
FAU - Shioi, T
AU  - Shioi T
FAU - Iwasaki, A
AU  - Iwasaki A
FAU - Matsushima, K
AU  - Matsushima K
FAU - Sasayama, S
AU  - Sasayama S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
RN  - 0 (Ion Channels)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.1.4.- (Type C Phospholipases)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics
MH  - Cytokines/metabolism
MH  - Endothelium, Vascular/*metabolism
MH  - Humans
MH  - Interleukin-8/*genetics
MH  - Ion Channels/metabolism
MH  - Physical Stimulation
MH  - Protein Kinase C/physiology
MH  - Protein-Tyrosine Kinases/physiology
MH  - RNA, Messenger/metabolism
MH  - Type C Phospholipases/physiology
MH  - *Up-Regulation
EDAT- 1998/06/20 00:00
MHDA- 1998/06/20 00:01
CRDT- 1998/06/20 00:00
PHST- 1998/06/20 00:00 [pubmed]
PHST- 1998/06/20 00:01 [medline]
PHST- 1998/06/20 00:00 [entrez]
AID - 10.1161/01.atv.18.6.894 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 1998 Jun;18(6):894-901. doi: 
      10.1161/01.atv.18.6.894.