PMID- 9633934
OWN - NLM
STAT- MEDLINE
DCOM- 19980708
LR  - 20190831
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 18
IP  - 6
DP  - 1998 Jun
TI  - IL-1beta-induced monocyte chemoattractant protein-1 gene expression in 
      endothelial cells is blocked by proteasome inhibitors.
PG  - 934-40
AB  - Human monocyte chemoattractant protein-1 (MCP-1) is expressed by a variety of 
      cell types in response to various stimuli. MCP-1 expressed by the endothelium 
      plays an important role in cell migration and activation. MCP-1 is a major 
      chemoattractant for monocytes, T lymphocytes, and basophils. In the present 
      study, we present evidence that the proteasome complex is involved in mediating 
      the interleukin (IL)-1beta induction of MCP-1 in endothelial cells. We present 
      evidence that a proteasome inhibitor, N-acetyl-leucinyl-leucinyl-norleucinal 
      (norLeu), and the protease inhibitor tosyl-Phe-chloromethylketone (TPCK) block 
      IL-1beta induction of MCP-1 protein expression. norLeu and TPCK also blocked 
      IL-1beta-induced MCP-1 promoter-driven reporter gene expression as well as 
      nuclear factor (NF)-kappaB-mediated reporter gene expression. The effects of 
      norLeu were due to its inhibition of the proteasome rather than calpain, because 
      other calpain inhibitors had no effect on MCP-1 expression. In contrast to TPCK, 
      which blocked NF-kappaB translocation to the nucleus, norLeu had no effect on 
      NF-kappaB nuclear translocation or IL-1beta-induced phosphorylation of p65. This 
      study demonstrates that the proteasome pathway is involved in IL-1beta-induced 
      MCP-1 gene expression in human endothelial cells.
FAU - Parry, G C
AU  - Parry GC
AD  - Department of Immunology, The Scripps Research Institute, La Jolla, Calif, USA.
FAU - Martin, T
AU  - Martin T
FAU - Felts, K A
AU  - Felts KA
FAU - Cobb, R R
AU  - Cobb RR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Interleukin-1)
RN  - 0 (Leupeptins)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (NF-kappa B)
RN  - 0 (Serine Proteinase Inhibitors)
RN  - 110044-82-1 (acetylleucyl-leucyl-norleucinal)
RN  - 402-71-1 (Tosylphenylalanyl Chloromethyl Ketone)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics
MH  - Cysteine Endopeptidases/*metabolism
MH  - Cysteine Proteinase Inhibitors/pharmacology
MH  - Endothelium, Vascular/*metabolism
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Interleukin-1/*physiology
MH  - Leupeptins/pharmacology
MH  - Multienzyme Complexes/*metabolism
MH  - NF-kappa B/metabolism
MH  - Proteasome Endopeptidase Complex
MH  - Serine Proteinase Inhibitors/*pharmacology
MH  - Tosylphenylalanyl Chloromethyl Ketone/pharmacology
EDAT- 1998/06/20 00:00
MHDA- 1998/06/20 00:01
CRDT- 1998/06/20 00:00
PHST- 1998/06/20 00:00 [pubmed]
PHST- 1998/06/20 00:01 [medline]
PHST- 1998/06/20 00:00 [entrez]
AID - 10.1161/01.atv.18.6.934 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 1998 Jun;18(6):934-40. doi: 
      10.1161/01.atv.18.6.934.