PMID- 9634645
OWN - NLM
STAT- MEDLINE
DCOM- 19980826
LR  - 20150831
IS  - 1043-6618 (Print)
IS  - 1043-6618 (Linking)
VI  - 37
IP  - 4
DP  - 1998 Apr
TI  - Inhibition of wound-induced expression of transforming growth factor-beta 1 mRNA 
      by its antisense oligonucleotides.
PG  - 289-93
AB  - Transforming growth factor-beta 1 (TGF-beta 1) is a member of a gene superfamily 
      that regulates proliferation, differentiation and other functions in many cell 
      types. To gain insight into the role of TGF-beta 1 in wound repair, we have 
      analysed the ability of an antisense TGF-beta 1 oligodeoxynucleotides (ODN) to 
      specifically inhibit wound-induced expression of TGF-beta 1 mRNA in the mouse 
      skin. Although injury induced the expression of TGF-beta 1 mRNA at the wound 
      sites, expression of TGF-beta 2-or TGF-beta 3-mRNA was not detected. In 
      comparison to the 24 h following injury, higher levels of TGF-beta 1 mRNA 
      expression were observed in the wound sites after 72 h. Northern blotting and in 
      situ hybridisation analysis showed that wound sites treated with antisense 
      TGF-beta 1 ODN exhibited no detectable levels of TGF-beta 1 mRNA after injury, 
      whereas the sites treated with sense TGF-beta 1 ODN possessed significant amounts 
      of its mRNA. Our results demonstrated that antisense TGF-beta 1 ODN inhibited the 
      wound-induced expression of TGF-beta 1 mRNA in vivo.
FAU - Kim, H M
AU  - Kim HM
AD  - Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, 
      South Korea.
FAU - Choi, D H
AU  - Choi DH
FAU - Lee, Y M
AU  - Lee YM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Pharmacol Res
JT  - Pharmacological research
JID - 8907422
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Depression, Chemical
MH  - In Situ Hybridization
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oligonucleotides, Antisense/*pharmacology
MH  - RNA Probes
MH  - RNA, Messenger/*biosynthesis/genetics/isolation & purification
MH  - Transforming Growth Factor beta/*biosynthesis/genetics
MH  - Wounds and Injuries/*metabolism
EDAT- 1998/06/23 00:00
MHDA- 1998/06/23 00:01
CRDT- 1998/06/23 00:00
PHST- 1998/06/23 00:00 [pubmed]
PHST- 1998/06/23 00:01 [medline]
PHST- 1998/06/23 00:00 [entrez]
AID - S1043-6618(98)90300-0 [pii]
AID - 10.1006/phrs.1998.0300 [doi]
PST - ppublish
SO  - Pharmacol Res. 1998 Apr;37(4):289-93. doi: 10.1006/phrs.1998.0300.