PMID- 9639493
OWN - NLM
STAT- MEDLINE
DCOM- 19980721
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 92
IP  - 1
DP  - 1998 Jul 1
TI  - Intracellular cytokine profile of cord and adult blood lymphocytes.
PG  - 11-8
AB  - Umbilical cord blood (CB) transplantation is thought to be associated with a 
      reduced risk of severe graft-versus-host-disease (GVHD) compared with bone marrow 
      transplantation (BMT). The cytokine cascade is known to be important in the 
      pathogenesis of GVHD; however, previous studies investigating the cytokine 
      secretion pattern of CB cells have been contradictory because of variations in 
      experimental techniques. In this study, the cytokine profile of cord and adult 
      blood lymphocytes and lymphocyte subsets has been assessed at the single-cell 
      level by flow cytometry, using CD4/CD8 and CD45RA/CD45RO markers. Cord and adult 
      blood mononuclear cells were stimulated with phorbol 12-myristate 13-acetate 
      (PMA) and ionomycin in the presence of monensin. After 4 to 24 hours of 
      incubation, interleukin-2 (IL-2), IL-4, interferon-gamma (IFN-gamma), and tumor 
      necrosis factor-alpha (TNF-alpha) production was measured by three-color flow 
      cytometry. The results show that cord blood lymphocytes (CBL) produce less IL-2, 
      IL-4, IFN-gamma, and TNF-alpha than adult peripheral blood lymphocytes (ABL). 
      Further subset analysis showed that in CBL the majority of cytokine producing 
      cells were CD4(+)CD45RA+, whereas in ABL the cytokine-producing cells were both 
      CD4(+)CD45RO+ and CD8(+)CD45RO+. These results suggest that the reduced incidence 
      of GVHD in CB transplantation may partly due to the altered cytokine profile seen 
      in CBL.
FAU - Chalmers, I M
AU  - Chalmers IM
AD  - Department of Histocompatibility and Immunogenetics, North London Centre (NBS), 
      London, UK.
FAU - Janossy, G
AU  - Janossy G
FAU - Contreras, M
AU  - Contreras M
FAU - Navarrete, C
AU  - Navarrete C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
SB  - IM
CIN - Blood. 1999 Feb 1;93(3):1120-2. PMID: 10025988
CIN - Blood. 1999 May 15;93(10):3566-7. PMID: 10366255
MH  - Adult
MH  - Antigens, CD/immunology
MH  - Cytokines/analysis/*immunology
MH  - Fetal Blood/*immunology
MH  - Flow Cytometry
MH  - Graft vs Host Disease/immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Lymphocytes/*immunology
MH  - T-Lymphocyte Subsets/immunology
EDAT- 1998/06/25 00:00
MHDA- 1998/06/25 00:01
CRDT- 1998/06/25 00:00
PHST- 1998/06/25 00:00 [pubmed]
PHST- 1998/06/25 00:01 [medline]
PHST- 1998/06/25 00:00 [entrez]
AID - S0006-4971(20)30028-8 [pii]
PST - ppublish
SO  - Blood. 1998 Jul 1;92(1):11-8.