PMID- 9639655
OWN - NLM
STAT- MEDLINE
DCOM- 19980915
LR  - 20190831
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 107
IP  - 3
DP  - 1998 Jun
TI  - Ordered arrangement and rearrangement of chromosomes during spermatogenesis in 
      two species of planarians (Plathelminthes).
PG  - 173-83
AB  - The pattern of distribution of telomeric DNA (TTAGGG), 28S rDNA, and 5S rDNA has 
      been studied using fluorescence in situ hybridization (FISH) and primed in situ 
      labelling during spermatogenesis and sperm formation in the filiform spermatozoa 
      of two species of planarians, Dendrocoelum lacteum and Polycelis tenuis 
      (Turbellaria, Plathelminthes). In both species, the positions of FISH signals 
      found with each probe sequence are constant from cell to cell in the nuclei of 
      mature sperm. Chromosome regions containing 5S and 28S rDNA genes are gathered in 
      distinct bundles of spiral form. In early spermatids with roundish nuclei, the 
      sites of a given sequence on different chromosomes remain separate. Centromeres 
      (marked by 5S rDNA) gather into a single cluster in the central region of the 
      slightly elongated sperm nucleus. During spermatid maturation, this cluster 
      migrates to the distal pole of the nucleus. In Polycelis, telomeric sites gather 
      into three distinct clusters at both ends and in the middle of the moderately 
      elongated nucleus. These clusters retain their relative positions as the 
      spermatid matures. All the chromosome ends bearing 28S rDNA gather only into the 
      proximal cluster. Our data suggest that structures in the nucleus selectively 
      recognise chromosome regions containing specific DNA sequences, which helps these 
      regions to find their regular places in the mature sperm nucleus and causes 
      clustering of the sites of these sequences located on different chromosomes. This 
      hypothesis is supported by observations on elongated sperm of other animals in 
      which a correlation exists between ordered arrangement of chromosomes in the 
      mature sperm nucleus and clustering of sites of the same sequence from different 
      chromosomes during spermiogenesis.
FAU - Joffe, B I
AU  - Joffe BI
AD  - Zoological Institute RAS, St Petersburg, Russia.
FAU - Solovei, I V
AU  - Solovei IV
FAU - Macgregor, H C
AU  - Macgregor HC
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 28S)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/ultrastructure
MH  - Chromosome Mapping
MH  - Chromosomes/chemistry/*genetics
MH  - DNA, Ribosomal/analysis/genetics
MH  - Gene Rearrangement/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Planarians/chemistry/*genetics
MH  - RNA, Ribosomal, 28S/analysis/genetics
MH  - RNA, Ribosomal, 5S/analysis/genetics
MH  - Repetitive Sequences, Nucleic Acid
MH  - Species Specificity
MH  - Spermatids/cytology/ultrastructure
MH  - Spermatogenesis/*genetics
MH  - Spermatozoa/cytology/ultrastructure
MH  - Telomere/chemistry/genetics
EDAT- 1998/06/26 00:00
MHDA- 1998/06/26 00:01
CRDT- 1998/06/26 00:00
PHST- 1998/06/26 00:00 [pubmed]
PHST- 1998/06/26 00:01 [medline]
PHST- 1998/06/26 00:00 [entrez]
AID - 10.1007/s004120050294 [doi]
PST - ppublish
SO  - Chromosoma. 1998 Jun;107(3):173-83. doi: 10.1007/s004120050294.